<p>Microfluidic technology has seen remarkable advancements in recent years, drawing significant attention in the field of pharmaceutical sciences under the term “Pharm-Lab-on-a-Chip.” Its appeal lies in numerous advantages, including high throughput, rapid detection, minimal reagent usage, efficient analysis, and compact design. Accordingly, the microfluidic platform is a&#xa0;promising pharmaceutical analysis tool. This review covers the advancements and utilization of microfluidic chip technology in pharmaceutical and pharmacological analysis. Firstly, the basic structure of a microfluidic chip was introduced, along with the fabrication of microfluidic chips. Subsequently, the use of the microfluidic chip in pharmaceutical analysis was discussed, with particular attention paid&#xa0;to the separation and analysis of drug molecules on the chip and integrating a microfluidic chip with other techniques. Furthermore, the role of microfluidic chips in pharmacological analysis was explored, mainly focusing on applying chip-based models for assessing the effectiveness and safety of drugs.</p> Graphical abstract <p></p>