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<p>This study compared four extraction methods: aqueous method (AM), aqueous enzymatic method (AEM), aqueous method-NaHCO<sub>3</sub> (AM-NaHCO<sub>3</sub>), and aqueous method-NaCl (AM-NaCl) to evaluate the extraction yield, rheological properties, and stability of sunflower oleosomes. All extracted oleosomes formed oil-in-water emulsions. Notably, the AM-NaHCO<sub>3</sub> method differed significantly from the other methods (<i>p</i> &lt; 0.05). It achieved the highest yield (41.19%), the smallest particle size (d<sub>4,3</sub>=3.99&#xa0;μm), and the lowest polydispersity index (PDI, a measure of particle size uniformity). The alkaline environment increased the zeta potential to − 32.68 mV, which likely reduced aggregation by enhancing electrostatic repulsion. Oleosome proteins prepared via the AM‑NaHCO<sub>3</sub> showed superior solubility (48.34%), emulsifying activity (62.1 m<sup>2</sup>/g), and emulsion stability (89.01&#xa0;min) (<i>p</i> &lt; 0.05). Overall, this study provides a theoretical basis for obtaining high-yield sunflower oleosomes with favorable stability.</p>

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Modulating sunflower oleosome stability and rheological behavior via extraction methods

  • 期刊论文

  • 发表日期：

    2026年6月17日

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  • Mengzhen Guan,
  • Fusheng Chen,
  • Dingyang Lv,
  • Xiuhong Yin

摘要

This study compared four extraction methods: aqueous method (AM), aqueous enzymatic method (AEM), aqueous method-NaHCO3 (AM-NaHCO3), and aqueous method-NaCl (AM-NaCl) to evaluate the extraction yield, rheological properties, and stability of sunflower oleosomes. All extracted oleosomes formed oil-in-water emulsions. Notably, the AM-NaHCO3 method differed significantly from the other methods (p < 0.05). It achieved the highest yield (41.19%), the smallest particle size (d4,3=3.99 μm), and the lowest polydispersity index (PDI, a measure of particle size uniformity). The alkaline environment increased the zeta potential to − 32.68 mV, which likely reduced aggregation by enhancing electrostatic repulsion. Oleosome proteins prepared via the AM‑NaHCO3 showed superior solubility (48.34%), emulsifying activity (62.1 m2/g), and emulsion stability (89.01 min) (p < 0.05). Overall, this study provides a theoretical basis for obtaining high-yield sunflower oleosomes with favorable stability.