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<p>Red oncom is a traditional Indonesian fermented food characterized by a yellowish-pink color derived from carotenoids produced by <i>Neurospora</i> spp. This study developed and validated a UV–Vis spectrophotometric method for the determination of total carotenoids using an optimized solvent extraction approach. Rapid, cost-effective analytical approaches are critical for improving quality control and analytical access in resource-limited, small-scale food production systems. Among the solvents evaluated, dimethyl sulfoxide (DMSO) and an acetone: hexane (7:3) mixture ranked first and second, respectively, in terms of extraction efficiency, with optimal conditions achieved using 6 mL DMSO and 16 mL acetone: hexane for 0.5&#xa0;g of sample. The method showed good linearity (R² = 0.997), acceptable accuracy (recoveries ranging from 83.3 to 92.1%), and satisfactory precision (relative standard deviation values of 2.29–4.98%), complying with AOAC and ICH Q2(R1) criteria. The limit of detection and quantification were 0.094&#xa0;µg/g and 0.285&#xa0;µg/g, respectively. The proposed method provides a rapid, simple, and cost-effective approach for routine carotenoid analysis in red oncom. In conclusion, the extraction and quantification methods for carotenoids in red oncom were successfully validated.</p>

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Methodological development and validation of quantitative analysis of carotenoid from red oncom as rapid analysis by UV-Vis spectrophotometry

  • 期刊论文

  • 发表日期：

    2026年4月20日

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  • Beneficia Rosy Acca,
  • Christofora Hanny Wijaya,
  • Lilis Nuraida,
  • Tatas Brotosudarmo,
  • Joncer Naibaho,
  • Sugiyono

摘要

Red oncom is a traditional Indonesian fermented food characterized by a yellowish-pink color derived from carotenoids produced by Neurospora spp. This study developed and validated a UV–Vis spectrophotometric method for the determination of total carotenoids using an optimized solvent extraction approach. Rapid, cost-effective analytical approaches are critical for improving quality control and analytical access in resource-limited, small-scale food production systems. Among the solvents evaluated, dimethyl sulfoxide (DMSO) and an acetone: hexane (7:3) mixture ranked first and second, respectively, in terms of extraction efficiency, with optimal conditions achieved using 6 mL DMSO and 16 mL acetone: hexane for 0.5 g of sample. The method showed good linearity (R² = 0.997), acceptable accuracy (recoveries ranging from 83.3 to 92.1%), and satisfactory precision (relative standard deviation values of 2.29–4.98%), complying with AOAC and ICH Q2(R1) criteria. The limit of detection and quantification were 0.094 µg/g and 0.285 µg/g, respectively. The proposed method provides a rapid, simple, and cost-effective approach for routine carotenoid analysis in red oncom. In conclusion, the extraction and quantification methods for carotenoids in red oncom were successfully validated.