Metabolomics and sensory profiling reveal flavor dynamics and bioactive evolution during jujube leaf tea blackening
摘要
This study elucidates the dynamic evolution of flavor and bioactive compounds in jujube leaf tea during 6 days blackening process under controlled conditions (50 °C, 65% relative humidity). Integrated analytical techniques, including high-performance liquid chromatography (HPLC), gas chromatography-tandem mass spectrometry (GC–MS), and electronic nose (E-nose), were employed to map the comprehensive quality transformation. Key findings revealed that gamma-aminobutyric acid (GABA) progressively accumulated, reaching 0.29 mg/g by day 6. Volatile profiling identified 56 aroma compounds dominated by ketones (31.4%) and alcohols (19.3%), with linalool increasing by 58.8%, correlating with sensory shifts from floral freshness to smoky richness. Organic acid analysis highlighted rising malic and lactic acids, contributing to the mellow and sour-bitter profile. Concurrently, significant changes in catechin profiles and free amino acids were observed, underpinning the taste evolution. Sensory evaluation confirmed the alignment of chemical changes with the development of a balanced smoky, sweet, and acidic flavor profile in late-stage infusions. These findings provide insights into the biochemical transformations, suggesting the potential synergistic roles of microbial metabolism, enzymatic reactions, and non-enzymatic thermal oxidations during blackening establishing a scientific foundation for optimizing processing protocols, enhancing nutraceutical value, and advancing sustainable utilization of agricultural byproducts. Ultimately, these findings establish a standardized, science-based protocol for industrial upscaling, enabling the transformation of post-harvest waste into a high-value functional beverage to support the circular bioeconomy and diversify regional agricultural income streams.