Scholarlink

<p>A high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method was developed for the determination of 1,3-dihydroxyacetone (DHA) in honey using the derivatization reagent, 2,4-dinitrophenylhydrazine. The method demonstrated high sensitivity, achieving a detection limit of 0.5&#xa0;mg/kg. The total analysis time for each sample, encompassing both preparation and instrumental analysis, was within 40&#xa0;min. Furthermore, the method exhibited good accuracy with an average recovery ranging from 84.5% to 104.7%, as well as satisfactory precision characterized by relative standard deviations below 4.8%. The method was applied to 201 honey samples from 25 botanical origins and 18 syrup samples of five types. The results demonstrated that the average DHA content in syrups of 56.32&#xa0;mg/kg was significantly higher than that in honey samples of 2.21&#xa0;mg/kg, except for Manuka honey of 293.52&#xa0;mg/kg. These findings suggest that DHA can serve as a potential marker for detecting honey adulteration, although this marker is not applicable to Manuka honey.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Determination of 1,3-Dihydroxyacetone in honey using 2,4-dinitrophenylhydrazine derivatization coupled with HPLC and its application in detecting adulteration

  • 期刊论文

  • 发表日期：

    2026年2月17日

查看全文
  • Jinyuan Wang,
  • Tiehong Zu,
  • Lili Dai,
  • Xiaomao Liu,
  • Xuezhe Huang,
  • Guangqun Jia,
  • Zongyan Cui,
  • Adan Li

摘要

A high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method was developed for the determination of 1,3-dihydroxyacetone (DHA) in honey using the derivatization reagent, 2,4-dinitrophenylhydrazine. The method demonstrated high sensitivity, achieving a detection limit of 0.5 mg/kg. The total analysis time for each sample, encompassing both preparation and instrumental analysis, was within 40 min. Furthermore, the method exhibited good accuracy with an average recovery ranging from 84.5% to 104.7%, as well as satisfactory precision characterized by relative standard deviations below 4.8%. The method was applied to 201 honey samples from 25 botanical origins and 18 syrup samples of five types. The results demonstrated that the average DHA content in syrups of 56.32 mg/kg was significantly higher than that in honey samples of 2.21 mg/kg, except for Manuka honey of 293.52 mg/kg. These findings suggest that DHA can serve as a potential marker for detecting honey adulteration, although this marker is not applicable to Manuka honey.