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<p>Live bacteria referred to as probiotics improve the health of their host when administered in adequate amounts. <i>Lactobacillus paracasei</i> (627) was investigated for in vitro screening and characterization for its usage in dragon fruit juice. Assays for in vitro screening were conducted, including those that assessed the epithelial barrier’s function, low pH, bile salt, adhesion capacity to human intestinal cell lines, impact on proteolytic activity, and transit tolerance in the upper gastrointestinal tract of humans. The strain demonstrated the ability to withstand high acidic conditions up to pH 2, as well as a reduction in viability from 8.6 to 7.4 log <i>cfu</i>/ml when exposed to up to 2% of bile salt concentration for 0 and 2&#xa0;h. For 0 and 2&#xa0;h, the isolates were able to withstand development against gastrointestinal juice at pH 2 and pH 2.5. It was discovered that hydrophobicity against xylene and toluene was 20 and 23.83%, respectively. Proteolytic activity clearance zone was found to be 12.4&#xa0;mm in skim milk agar assay. This study illustrates how strain-specific factors affect vitro screening procedures. <i>Lactobacillus paracasei (627)</i> strain showcased good results after incorporating in dragon fruit juice and provided 14 days of shelf life, with the acceptable limit of probiotic count.</p>

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Exploring the potential: in vitro analysis of Lactobacillus paracasei (627) and its application in enhancing Hylocerus polyrhizus (Dragon fruit) juice quality

  • 期刊论文

  • 发表日期：

    2026年2月6日

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  • Mansi Kshirsagar,
  • Gauri Harish Athawale,
  • Raichurkar S. J

摘要

Live bacteria referred to as probiotics improve the health of their host when administered in adequate amounts. Lactobacillus paracasei (627) was investigated for in vitro screening and characterization for its usage in dragon fruit juice. Assays for in vitro screening were conducted, including those that assessed the epithelial barrier’s function, low pH, bile salt, adhesion capacity to human intestinal cell lines, impact on proteolytic activity, and transit tolerance in the upper gastrointestinal tract of humans. The strain demonstrated the ability to withstand high acidic conditions up to pH 2, as well as a reduction in viability from 8.6 to 7.4 log cfu/ml when exposed to up to 2% of bile salt concentration for 0 and 2 h. For 0 and 2 h, the isolates were able to withstand development against gastrointestinal juice at pH 2 and pH 2.5. It was discovered that hydrophobicity against xylene and toluene was 20 and 23.83%, respectively. Proteolytic activity clearance zone was found to be 12.4 mm in skim milk agar assay. This study illustrates how strain-specific factors affect vitro screening procedures. Lactobacillus paracasei (627) strain showcased good results after incorporating in dragon fruit juice and provided 14 days of shelf life, with the acceptable limit of probiotic count.