Colorimetric quantitative detection for saccharinity and protein content in protein beverage based on patterned wettability surface
摘要
The colorimetric quantitative detection method was a high-efficient on-site test technology. So, the colorimetric quantitative detection method was developed for simultaneously testing the glucose, sucrose and protein contents in protein beverages, which was based on the patterned wettability surfaces, image acquiring and image processing. The glucose detection range was 5–50 mM (90–900 mg/100mL) with detection limit of 0.746 mM (13.44 mg/100mL). The sucrose detection range was 15–200 mM (513–6840 mg/100mL) with detection limit of 0.0626 mM (2.14 mg/100mL). The protein detection range was 0–75 µM (0–510 mg/100mL) with detection limit of 0.187 µM (1.27 mg/100mL). Besides, all the relative standard deviations of the saccharides and protein test results were less than 1%. The gray values of the interference were at most accounted for 35.66% in those of the target glucose, sucrose and protein. The t1/2 of colorimetric test chip could be stored up to 236 days at 4 ℃. The colorimetric method was as the same precise as the ultraviolet method. So, the colorimetric quantitative detection method was precise and stable. An efficient and portable method for simultaneously testing saccharinity and protein content in protein beverages was provided.