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<p>In this study, 18 different extracts from Moroccan <i>Cannabis sativa</i> L. (resin, leaves, and seeds) were screened for their phytochemical composition, antioxidant, and antimicrobial properties. The ethanol–water (7:3) extract from resin and the methanol extract from leaves revealed high total polyphenol contents (TPC), with 65.41 ± 6.37 and 59.70 ± 5.40 mg GAE/g, while the methanol extract from leaves had the highest value of total flavonoid contents (TFC) with 11.47 ± 0.25 mg QuE/g. The antioxidant activities were evaluated using four different methods including the DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging assay, FRAP (ferric reducing antioxidant potential) assay, TAC (total antioxidant capacity) assay, and <i>β</i>-carotene bleaching assay. The IC<sub>50</sub> values obtained ranged between 49.70 ± 1.14 and 453.60 ± 10.14 μg/mL for DPPH, 103.58 ± 5.18 and 738.21 ± 36.91 μg/mL for FRAP, 101.96 ± 5.10 and 1956.40 ± 97.82 μg/mL for TAC, and 205.57 ± 5.37 and 797.02 ± 21.84 μg/mL for <i>β</i>-carotene bleaching assay. The antimicrobial activities were evaluated against <i>Enterococcus faecalis</i> S5<i>, Staphylococcus aureus</i> B804, <i>Pseudomonas fragi</i> S23, <i>Escherichia coli</i> DH5α, <i>Salmonella enterica</i> B801, and <i>Listeria monocytogenes</i> B806. All extracts showed higher activity against <i>S. aureus</i> (DIZ = 9–19.5 mm, MIC = 0.031–8 mg/mL)<i>.</i> The LC–MS analysis allowed the identification of 19 phytocannabinoids in ethanol–water (7:3) extract from resin highlighting the remarkable phytochemical richness of the Moroccan <i>Cannabis sativa</i> (Beldia) cultivar. The principal component analysis and Pearson correlation matrix plot showed a strong correlation between the antioxidant activities measured by DPPH and FRAP assays (r = 0.85). Finally, the observed antimicrobial and antioxidant activities indicate that these extracts may serve as natural antimicrobial agents and as dietary supplements to prevent oxidative stress.</p>

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Total polyphenol contents, total flavonoid contents, LC–MS analysis, antioxidant, and antimicrobial activities of Moroccan Cannabis sativa L. (Beldia): A comparative study of resin, leaf, and seed extracts and chemometric approach

  • 期刊论文

  • 发表日期：

    2026年3月3日

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  • Héritier Uwikunda Serondo,
  • Meryem Bouraqqadi,
  • Hamid El Amri,
  • Samir Ananou,
  • Saïd El Kazzouli,
  • Nabil El Brahmi

摘要

In this study, 18 different extracts from Moroccan Cannabis sativa L. (resin, leaves, and seeds) were screened for their phytochemical composition, antioxidant, and antimicrobial properties. The ethanol–water (7:3) extract from resin and the methanol extract from leaves revealed high total polyphenol contents (TPC), with 65.41 ± 6.37 and 59.70 ± 5.40 mg GAE/g, while the methanol extract from leaves had the highest value of total flavonoid contents (TFC) with 11.47 ± 0.25 mg QuE/g. The antioxidant activities were evaluated using four different methods including the DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging assay, FRAP (ferric reducing antioxidant potential) assay, TAC (total antioxidant capacity) assay, and β-carotene bleaching assay. The IC50 values obtained ranged between 49.70 ± 1.14 and 453.60 ± 10.14 μg/mL for DPPH, 103.58 ± 5.18 and 738.21 ± 36.91 μg/mL for FRAP, 101.96 ± 5.10 and 1956.40 ± 97.82 μg/mL for TAC, and 205.57 ± 5.37 and 797.02 ± 21.84 μg/mL for β-carotene bleaching assay. The antimicrobial activities were evaluated against Enterococcus faecalis S5, Staphylococcus aureus B804, Pseudomonas fragi S23, Escherichia coli DH5α, Salmonella enterica B801, and Listeria monocytogenes B806. All extracts showed higher activity against S. aureus (DIZ = 9–19.5 mm, MIC = 0.031–8 mg/mL). The LC–MS analysis allowed the identification of 19 phytocannabinoids in ethanol–water (7:3) extract from resin highlighting the remarkable phytochemical richness of the Moroccan Cannabis sativa (Beldia) cultivar. The principal component analysis and Pearson correlation matrix plot showed a strong correlation between the antioxidant activities measured by DPPH and FRAP assays (r = 0.85). Finally, the observed antimicrobial and antioxidant activities indicate that these extracts may serve as natural antimicrobial agents and as dietary supplements to prevent oxidative stress.