Study on the changes in curcuma components and antioxidant activity at different cooking times based on untargeted metabolomics
摘要
To compare the compositional changes of turmeric at different cooking times and to provide a theoretical basis for the development of turmeric-based medicinal and food products. Methods: Fresh turmeric was boiled for 1 h and 2 h, respectively, and non-targeted metabolomics was used to analyze the differences in metabolites in turmeric subjected to different boiling treatments. The color and luster of turmeric subjected to different treatments were analyzed using a colorimeter, and the antioxidant capacity of turmeric with different treatments was determined by the DPPH method and the T-AOC method. The results showed that a total of 2,130 metabolites were identified in turmeric subjected to different treatments, and 117 key metabolites were screened by VIP > 1 and p < 0.05. Six new substances were produced after cooking: ginger glycolipid C, paxillin, isoscoparin 2’’-(6-(E)-feruloylglucoside) 4’-glucoside, abscisic acid ester, phosphatidylethanolamine, and 5-methoxy-8-(1,1-dimethyl-2,3-dihydroxypropyl)-psoralen. In addition, there was a significant increase in the relative content of these metabolites with increasing cooking time. The L*, a*, and b* values of turmeric after cooking treatment showed a decreasing trend. The L* value of fresh turmeric decreased from 43.7 ± 0.27 to 41.8 ± 0.34, the a* value from 6.5 ± 0.17 to 6.0 ± 0.24, and the b* value from 19.0 ± 0.51 to 15.9 ± 0.52 with the extension of cooking time. The antioxidant capacity of turmeric treated with boiling was measured using the DPPH method and the T-AOC method. The antioxidant capacity of the treated turmeric was found to be lowest in fresh samples (63.35%±0.37) by the DPPH method, increased significantly after 1 h (74.46%±2.89), but decreased significantly at 2 h (70.38%±0.12).The antioxidant capacity of the fresh samples measured by the T-AOC method showed more or less the same trend as that of the DPPH method. The lowest antioxidant capacity was observed in the fresh sample (3.03 ± 0.01 µmol/g), with a significant enhancement in antioxidant capacity at 1 h (5.68 ± 0.01 µmol/g), and a further increase in antioxidant capacity with prolonged cooking time at 2 h (5.83 ± 0.01 µmol/g).Conclusion: Cooking treatment alters the chemical composition of turmeric, its colour deepens with prolonged cooking time, and its antioxidant capacity is improved. This study provides a theoretical basis for the development of medicinal and food products derived from turmeric.