Antioxidant properties, phytochemical characterization and quantification of sugars, HMF, pesticides by liquid chromatography in Moroccan monofloral honey
摘要
Honey has been used for centuries as both food and medicine. This study examines and compares Brix, sugar content, hydroxymethylfurfural (HMF) content, pesticide contamination, and antioxidant properties of eight types of Moroccan monofloral honey. HPLC was employed to analyze the honey samples for their sugar composition and HMF levels and to identify any pesticide residues. The total antioxidant capacity of honey was assessed using three assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP). The results were statistically analyzed. Honey samples met criteria established by the European Union and showed antioxidant potential. They exhibited significant polyphenolic (24.30–99.24 mg EqAA/100 g) and flavonoid content (8.64–29.58 mg EqQ/100 g), with p < 0.05. The correlations between antioxidant results and polyphenol content were statistically significant. This study presents preliminary findings on the phenolic profile and total antioxidant capacity of these Moroccan monofloral honey. In addition, no pesticides residues were found, confirming their purity and safety. Data indicates its monofloral origins and its potential as a natural source of antioxidants with significant medical properties. It may be utilized as a natural nutraceutical for the management of diseases associated with free radical damaging.