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<p>In this work, the purity of polyphenols in brown mustard seeds extract (BMSE) was improved by macroporous resin screening, adsorption kinetics investigation, and the optimization of dynamic adsorption/desorption parameters. AB-8 was identified as the optimal resin. Optimal adsorption/desorption parameters were 2&#xa0;mg/mL sample concentration, 40&#xa0;mL sample volume, and desorption with 60% ethanol, at which conditions, the purity of polyphenols from purified brown mustard seeds extract (BMSE-P) was 5.64-fold higher than BMSE. Nine phenolics and six flavonoids were detected in BMSE-P through LC–MS/MS analysis. Moreover, BMSE-P exhibited superior in vitro antioxidant activity. Further exploration of the radioprotective activity of BMSE-P showed that BMSE-P could promote the proliferation and effectively inhibit the ROS generation of <sup>60</sup>Coγ radiation-damaged HL-7702 cells. This work could establish a basis for purifying brown mustard seeds polyphenols and advancing their further application in functional food.</p>

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Polyphenols from brown mustard seeds (Brassica juncea) extract, purification, antioxidant and radioprotective activities in vitro

  • 期刊论文

  • 发表日期：

    2025年10月4日

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  • Shiyu Zhao,
  • Yue Zhang,
  • Xue Li,
  • Jike Lu,
  • Juanjuan Yi,
  • Xin Liu,
  • Jiaqing Zhu,
  • Qiaozhen Kang,
  • Yanling Shi

摘要

In this work, the purity of polyphenols in brown mustard seeds extract (BMSE) was improved by macroporous resin screening, adsorption kinetics investigation, and the optimization of dynamic adsorption/desorption parameters. AB-8 was identified as the optimal resin. Optimal adsorption/desorption parameters were 2 mg/mL sample concentration, 40 mL sample volume, and desorption with 60% ethanol, at which conditions, the purity of polyphenols from purified brown mustard seeds extract (BMSE-P) was 5.64-fold higher than BMSE. Nine phenolics and six flavonoids were detected in BMSE-P through LC–MS/MS analysis. Moreover, BMSE-P exhibited superior in vitro antioxidant activity. Further exploration of the radioprotective activity of BMSE-P showed that BMSE-P could promote the proliferation and effectively inhibit the ROS generation of 60Coγ radiation-damaged HL-7702 cells. This work could establish a basis for purifying brown mustard seeds polyphenols and advancing their further application in functional food.