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<p>The pupal shells of the black soldier fly (BSF) show great potential in the production of chitin and chitosan. Compared to the traditional chemical acid–base deproteinization method, this study was devoted to the development of a more environmentally friendly enzymatic deproteinization technique to extract higher quality chitosan. Response surface methodology (RSM) was used to determine the optimal conditions for enzymatic hydrolysis in terms of reaction time (3.2&#xa0;h), reaction temperature (49&#xa0;°C) and enzyme/substrate ratio (5.4:100). The physicochemical properties of chitosan CS<sub>E</sub> obtained by protease hydrolysis and chitosan CS<sub>C</sub> obtained by traditional acid–base method were determined and chitosan samples CS<sub>E</sub> and CS<sub>C</sub> were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). The results showed that the yield of CS<sub>E</sub> was significantly higher at 18.85 ± 0.02% than that of CS<sub>C</sub> at 11.29 ± 0.03%. Chitosan extracted from BSF pupal shells by enzymatic deproteinization showed antimicrobial activity against all tested bacterial strains, with relatively high antibacterial activity against <i>Staphylococcus aureus</i> (14.41 ± 0.54&#xa0;mm), <i>Escherichia coli</i> (18.01 ± 0.66&#xa0;mm). The superior yield and antimicrobial properties of chitosan extracted via enzymatic deproteinization suggest broad potential applications beyond biomedical sciences, including food preservation and water treatment fields where antimicrobial agents are highly sought. Future research is recommended to explore these applications, thereby maximizing the benefits of this environmentally friendly extraction method.</p>

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Chitosan extracted from pupal shells of black soldier fly by two deproteinization methods and its characterization

  • 期刊论文

  • 发表日期：

    2025年9月26日

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  • Yirong Tao,
  • Qiang Xiong,
  • Zeyi Zhang,
  • Yun Sun,
  • Jingjun Li,
  • Xinfu Li

摘要

The pupal shells of the black soldier fly (BSF) show great potential in the production of chitin and chitosan. Compared to the traditional chemical acid–base deproteinization method, this study was devoted to the development of a more environmentally friendly enzymatic deproteinization technique to extract higher quality chitosan. Response surface methodology (RSM) was used to determine the optimal conditions for enzymatic hydrolysis in terms of reaction time (3.2 h), reaction temperature (49 °C) and enzyme/substrate ratio (5.4:100). The physicochemical properties of chitosan CSE obtained by protease hydrolysis and chitosan CSC obtained by traditional acid–base method were determined and chitosan samples CSE and CSC were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). The results showed that the yield of CSE was significantly higher at 18.85 ± 0.02% than that of CSC at 11.29 ± 0.03%. Chitosan extracted from BSF pupal shells by enzymatic deproteinization showed antimicrobial activity against all tested bacterial strains, with relatively high antibacterial activity against Staphylococcus aureus (14.41 ± 0.54 mm), Escherichia coli (18.01 ± 0.66 mm). The superior yield and antimicrobial properties of chitosan extracted via enzymatic deproteinization suggest broad potential applications beyond biomedical sciences, including food preservation and water treatment fields where antimicrobial agents are highly sought. Future research is recommended to explore these applications, thereby maximizing the benefits of this environmentally friendly extraction method.