Scholarlink

<p>This study compared ultrasonic-assisted extraction (UAE) with conventional methods for the extraction of glucomoringin from Moringa oleifera seeds. Response Surface Methodology (RSM) was employed to examine the effects of three factors on the yield of the bioactive molecule glucomoringin: extraction period (15–25&#xa0;min), extraction temperature (40–60&#xa0;°C), and the sample-to-solvent ratio of 1:5 − 1:10. The optimal conditions were established as 15&#xa0;min at 60&#xa0;°C with a sample to solvent ratio of 1:5, yielding 4.244&#xa0;mg/g of glucomoringin in moringa seeds. Further, ultrasonic-assisted extraction (UAE) methodology revealed significantly higher efficiency, producing approximately four times more glucomoringin than the conventional soaking method (0.992&#xa0;mg/g of glucomoringin). A quadratic model (<i>p</i> &lt; 0.05) was developed to forecast glucomoringin production, with a strong fit (R² = 0.9465). Additionally, the study revealed that the UAE significantly enhanced glucomoringin extraction efficiency and reduced processing time relative to conventional techniques. The phytochemical analysis indicated that the methanolic extract had flavonoids, alkaloids, and saponins. Quantitative analysis revealed concentrations of 246.93&#xa0;µg/ml flavonoids, 191.35&#xa0;µg/ml alkaloids, and 307.24&#xa0;µg/ml saponins in the extract. The extracts demonstrated considerable antioxidant action. At a concentration of 250&#xa0;µg/ml, the extract exhibited 59% inhibition of DPPH; 57.5% suppression of hydrogen peroxide, and 69.5% scavenging activity against hydroxyl radicals. This research highlighted the efficacy of ultrasonic-assisted extraction in extracting bioactive glucosinolates from <i>Moringa oleifera</i> seeds. The isolated compounds exhibited notable antioxidant properties, suggesting their prospective use in nutraceuticals, functional foods, and medicines.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optimization of ultrasonic-assisted extraction of Glucomoringin from Moringa oleifera seeds: process parameters, phytochemical characterization, and antioxidant activity

  • 期刊论文

  • 发表日期：

    2025年9月20日

查看全文
  • B. Ravi Prakash,
  • R. Arulmari,
  • I. P. Sudagar,
  • K. Gurusamy,
  • R. Surya Priyadharshini,
  • G. G. Kavitha Shree,
  • P. Rajkumar,
  • P. Preetha,
  • R. Pandiselvam

摘要

This study compared ultrasonic-assisted extraction (UAE) with conventional methods for the extraction of glucomoringin from Moringa oleifera seeds. Response Surface Methodology (RSM) was employed to examine the effects of three factors on the yield of the bioactive molecule glucomoringin: extraction period (15–25 min), extraction temperature (40–60 °C), and the sample-to-solvent ratio of 1:5 − 1:10. The optimal conditions were established as 15 min at 60 °C with a sample to solvent ratio of 1:5, yielding 4.244 mg/g of glucomoringin in moringa seeds. Further, ultrasonic-assisted extraction (UAE) methodology revealed significantly higher efficiency, producing approximately four times more glucomoringin than the conventional soaking method (0.992 mg/g of glucomoringin). A quadratic model (p < 0.05) was developed to forecast glucomoringin production, with a strong fit (R² = 0.9465). Additionally, the study revealed that the UAE significantly enhanced glucomoringin extraction efficiency and reduced processing time relative to conventional techniques. The phytochemical analysis indicated that the methanolic extract had flavonoids, alkaloids, and saponins. Quantitative analysis revealed concentrations of 246.93 µg/ml flavonoids, 191.35 µg/ml alkaloids, and 307.24 µg/ml saponins in the extract. The extracts demonstrated considerable antioxidant action. At a concentration of 250 µg/ml, the extract exhibited 59% inhibition of DPPH; 57.5% suppression of hydrogen peroxide, and 69.5% scavenging activity against hydroxyl radicals. This research highlighted the efficacy of ultrasonic-assisted extraction in extracting bioactive glucosinolates from Moringa oleifera seeds. The isolated compounds exhibited notable antioxidant properties, suggesting their prospective use in nutraceuticals, functional foods, and medicines.

Graphical abstract