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<p>Pulsed light (PL) treatment is a promising non-thermal technology for ensuring the safety and quality of food and water. Its effectiveness in liquids, however, varies due to challenges such as reduced light penetration and uneven exposure, especially in larger volumes. Effective mixing and optimized treatment durations are therefore crucial for enhancing PL’s efficiency. In this study, sterile water inoculated with <i>Escherichia coli</i> K-12 (EC1-5G) underwent various PL treatments to evaluate the impact of treatment duration, agitation speed, and sample volume on microbial inactivation. A first-order kinetic model was applied to calculate D-values (time required for a 90% reduction in the microbial population). At a constant sample volume of 400 mL, increasing agitation speed significantly decreased D-values, from 6.06&#xa0;s under static conditions (0&#xa0;rpm) to 2.96&#xa0;s at 1,000&#xa0;rpm. Conversely, increasing sample volume at a constant agitation speed of 500&#xa0;rpm resulted in a significant rise in D-values, from 0.63&#xa0;s for 100 mL to 4.40&#xa0;s for 400 mL, emphasizing the need for longer treatment times in larger volumes. Interestingly, treating multiple containers simultaneously resulted in microbial inactivation levels comparable to those observed in single-container treatments. The findings underscore the importance of optimizing agitation speed and using smaller volumes or simultaneously treating multiple containers to enhance PL decontamination efficiency. These strategies ensure effective microbial reduction while maintaining operational efficiency. This study highlights the potential of PL as a scalable and energy-efficient method for drinking water treatment as well as liquid food processing, providing a reliable solution for microbial inactivation across varying volumes and conditions.</p>

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Enhanced inactivation of Escherichia coli in water using agitation-assisted pulsed light treatment

  • 期刊论文

  • 发表日期：

    2025年9月12日

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  • Bosco Mategeko,
  • Hosahalli S. Ramaswamy

摘要

Pulsed light (PL) treatment is a promising non-thermal technology for ensuring the safety and quality of food and water. Its effectiveness in liquids, however, varies due to challenges such as reduced light penetration and uneven exposure, especially in larger volumes. Effective mixing and optimized treatment durations are therefore crucial for enhancing PL’s efficiency. In this study, sterile water inoculated with Escherichia coli K-12 (EC1-5G) underwent various PL treatments to evaluate the impact of treatment duration, agitation speed, and sample volume on microbial inactivation. A first-order kinetic model was applied to calculate D-values (time required for a 90% reduction in the microbial population). At a constant sample volume of 400 mL, increasing agitation speed significantly decreased D-values, from 6.06 s under static conditions (0 rpm) to 2.96 s at 1,000 rpm. Conversely, increasing sample volume at a constant agitation speed of 500 rpm resulted in a significant rise in D-values, from 0.63 s for 100 mL to 4.40 s for 400 mL, emphasizing the need for longer treatment times in larger volumes. Interestingly, treating multiple containers simultaneously resulted in microbial inactivation levels comparable to those observed in single-container treatments. The findings underscore the importance of optimizing agitation speed and using smaller volumes or simultaneously treating multiple containers to enhance PL decontamination efficiency. These strategies ensure effective microbial reduction while maintaining operational efficiency. This study highlights the potential of PL as a scalable and energy-efficient method for drinking water treatment as well as liquid food processing, providing a reliable solution for microbial inactivation across varying volumes and conditions.