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<p>Debranning results in the loss of peripheral layers (bran/seed coat, aleurone) and germ (G) and associated dietary fibre, proteins, minerals and phytochemicals in the kernel, leading to a concomitant increase in the starchy endosperm in debranned cereals. Such information is not available in millets. Millets (foxtail, little, kodo, barnyard and proso) in their dehusked (whole grain) and debranned (polished) forms were analysed for total phenolic contents. Debranning of millets resulted in a significant decrease in the total phenolic content in all millets. In addition, the millet sections were examined for autofluorescence, which visualized seed coat (SC), aleurone layer (AL), germ (G) and endosperm (E) architecture in a bright blue fluorescence. Debranning resulted in the loss of SC and AL but retained partial G. The areas of these botanical constituents were measured using ImageJ software and were used for computing E: G, E: G + SC + AL ratios. These ratios increased upon debranning in millets, indicating an increase in the E fraction. Starch and proteins in the millet sections were localized using iodine and FCF green stains. Starch was mainly concentrated in the E, while the proteins were distributed in the G, SC, AL, and E regions. Dehusked millets are healthier options in the context of non-communicable disease prevention.</p>

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Fluorescence microscopic and histochemical localization studies on dehusked and debranned Indian millets

  • 期刊论文

  • 发表日期：

    2025年8月8日

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  • S. Shobana,
  • B. Dayakar Rao,
  • K. Mohanraj,
  • N. G. Malleshi,
  • R. M. Anjana,
  • V. Mohan

摘要

Debranning results in the loss of peripheral layers (bran/seed coat, aleurone) and germ (G) and associated dietary fibre, proteins, minerals and phytochemicals in the kernel, leading to a concomitant increase in the starchy endosperm in debranned cereals. Such information is not available in millets. Millets (foxtail, little, kodo, barnyard and proso) in their dehusked (whole grain) and debranned (polished) forms were analysed for total phenolic contents. Debranning of millets resulted in a significant decrease in the total phenolic content in all millets. In addition, the millet sections were examined for autofluorescence, which visualized seed coat (SC), aleurone layer (AL), germ (G) and endosperm (E) architecture in a bright blue fluorescence. Debranning resulted in the loss of SC and AL but retained partial G. The areas of these botanical constituents were measured using ImageJ software and were used for computing E: G, E: G + SC + AL ratios. These ratios increased upon debranning in millets, indicating an increase in the E fraction. Starch and proteins in the millet sections were localized using iodine and FCF green stains. Starch was mainly concentrated in the E, while the proteins were distributed in the G, SC, AL, and E regions. Dehusked millets are healthier options in the context of non-communicable disease prevention.