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<p>The <i>Zingiber officinale Rosc.(ZOR)</i> is a widely used edible and medicinal herb in Eastern Asia, particularly in China, known for its numerous beneficial active ingredients. In this study, a simple, sensitive, precise, and specific reverse phase ultra-high-performance liquid chromatography with diode array detection (UPLC-DAD) method was developed to quantify four gingerols in <i>ZOR</i>, including 6-gingerol (6G), 8-gingerol (8G), 10-gingerol (10G), and 6-Shogaol (6S). Using this method, a quantitative analysis of multicomponents through a single marker (QAMS) was conducted. Relative correction factors (<i>RCFs</i>) were used to quantify gingerols, with capsaicin as an internal standard due to its low cost, availability, and structural/polarity similarity to the analytes. The accuracy of the QAMS method was verified by comparing its results with those from the external standard method (ED), demonstrating its feasibility and adaptability for quality control of <i>ZOR</i>. The four compounds were baseline separated in 25&#xa0;min with a high linearity of the regression coefficient (<i>R&gt;</i>0.9999). The accuracy of the QAMS method varied between 90.20% and 114.84%. Additionally, the relative standard deviation (RSD) values for both the <i>RCF</i> and relative retention time (<i>Rt</i><sub><i>R</i></sub>) across different injection volumes ranged from 1.14 to 4.15% and 0.04–0.06%, respectively. The QAMS results indicated that it is a convenient and accurate method for determining multicomponent, especially when authentic standard substances are unavailable. Notably, the results of antioxidant and antimicrobial activity evaluation revealed that ginger extract could effectively scavenge DPPH·, ·OH and ·O<sup>2−</sup> free radicals, showing strong antioxidant activity, and consistent with the antioxidant results, ginger extract also had a broad-spectrum antimicrobial effect, which may be related to the high content of gingerols, with significant quantitative and quantitative effect relationships. This can be further applied to the quality assessment and biofunctional evaluation of herbal medicines.</p>

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Novel UPLC-DAD approach for dual-mode quantification of gingerols in Zingiber officinale roscoe: integrating absolute quantification with QAMS and assessing biofunctional activities

  • 期刊论文

  • 发表日期：

    2025年8月29日

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  • Zhining Li,
  • Feifei Li,
  • Kai Fu,
  • Wenjing Bu,
  • Lulu Zhang,
  • Sai Zhang,
  • Yi Fan,
  • Xiao Li,
  • Chun Chang

摘要

The Zingiber officinale Rosc.(ZOR) is a widely used edible and medicinal herb in Eastern Asia, particularly in China, known for its numerous beneficial active ingredients. In this study, a simple, sensitive, precise, and specific reverse phase ultra-high-performance liquid chromatography with diode array detection (UPLC-DAD) method was developed to quantify four gingerols in ZOR, including 6-gingerol (6G), 8-gingerol (8G), 10-gingerol (10G), and 6-Shogaol (6S). Using this method, a quantitative analysis of multicomponents through a single marker (QAMS) was conducted. Relative correction factors (RCFs) were used to quantify gingerols, with capsaicin as an internal standard due to its low cost, availability, and structural/polarity similarity to the analytes. The accuracy of the QAMS method was verified by comparing its results with those from the external standard method (ED), demonstrating its feasibility and adaptability for quality control of ZOR. The four compounds were baseline separated in 25 min with a high linearity of the regression coefficient (R>0.9999). The accuracy of the QAMS method varied between 90.20% and 114.84%. Additionally, the relative standard deviation (RSD) values for both the RCF and relative retention time (RtR) across different injection volumes ranged from 1.14 to 4.15% and 0.04–0.06%, respectively. The QAMS results indicated that it is a convenient and accurate method for determining multicomponent, especially when authentic standard substances are unavailable. Notably, the results of antioxidant and antimicrobial activity evaluation revealed that ginger extract could effectively scavenge DPPH·, ·OH and ·O2− free radicals, showing strong antioxidant activity, and consistent with the antioxidant results, ginger extract also had a broad-spectrum antimicrobial effect, which may be related to the high content of gingerols, with significant quantitative and quantitative effect relationships. This can be further applied to the quality assessment and biofunctional evaluation of herbal medicines.