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<p><i>Psophocarpus tetragonolobus</i> (L.) DC seed (PT) is rich in protein and holds potential as a valuable nutritional supplement. To explore its antioxidant properties, protein from the winged bean seed (PTP) was extracted and hydrolyzed with alcalase to produce a protein hydrolysate (PTPH). The antioxidant activity of PTPH was evaluated by measuring its 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assay and 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging activities. It exhibited potent DPPH and ABTS scavenging activities, with IC<sub>50</sub> values of 2.10 ± 1.03 and 0.73 ± 2.01&#xa0;µg/mL, respectively. PTPH demonstrated no toxicity toward L929 mouse fibroblast cells and was cytoprotective against H<sub>2</sub>O<sub>2</sub>-induced damage in L929 cells. At low concentrations, it even promoted cell proliferation. Using anion exchange chromatography and liquid chromatography-mass spectrometry/mass spectrometry (LC–MS/MS), ten peptides with molecular weights ranging from 707.35 to 1,478.80&#xa0;Da were isolated and identified from PTPH. Three synthetic peptides (YGDPVKF, LSHTLFGDELCK, and YLYELAR) exhibited strong ABTS radical scavenging activity and protected L929 cells from H₂O₂-induced oxidative stress by reducing intracellular ROS levels. These findings suggest that the protein hydrolysate and peptides derived from PT seeds have potential applications as natural antioxidants in food supplements and health-related nutraceuticals.</p>

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Novel antioxidant and cytoprotective peptides from Psophocarpus tetragonolobus (L.) DC seed protein hydrolysate

  • 期刊论文

  • 发表日期：

    2025年3月10日

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  • Prapassorn Puenprom,
  • Anirut Hlosrichok,
  • Ratchaneewan Aunpad

摘要

Psophocarpus tetragonolobus (L.) DC seed (PT) is rich in protein and holds potential as a valuable nutritional supplement. To explore its antioxidant properties, protein from the winged bean seed (PTP) was extracted and hydrolyzed with alcalase to produce a protein hydrolysate (PTPH). The antioxidant activity of PTPH was evaluated by measuring its 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assay and 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging activities. It exhibited potent DPPH and ABTS scavenging activities, with IC50 values of 2.10 ± 1.03 and 0.73 ± 2.01 µg/mL, respectively. PTPH demonstrated no toxicity toward L929 mouse fibroblast cells and was cytoprotective against H2O2-induced damage in L929 cells. At low concentrations, it even promoted cell proliferation. Using anion exchange chromatography and liquid chromatography-mass spectrometry/mass spectrometry (LC–MS/MS), ten peptides with molecular weights ranging from 707.35 to 1,478.80 Da were isolated and identified from PTPH. Three synthetic peptides (YGDPVKF, LSHTLFGDELCK, and YLYELAR) exhibited strong ABTS radical scavenging activity and protected L929 cells from H₂O₂-induced oxidative stress by reducing intracellular ROS levels. These findings suggest that the protein hydrolysate and peptides derived from PT seeds have potential applications as natural antioxidants in food supplements and health-related nutraceuticals.