Extraction and electrochemical sensing of anthocyanins in berry fruits by use of carbon nanotube-based electrode

This study uses a modified electrode made of Molecularly Imprinted Polymers (MIPs) and Multi-Walled Carbon Nanotubes (MWCNTs) to provide a unique method for the extraction and electrochemical detection of cyanidin in berry fruits. The special pairing of MIPs with MWCNTs improves the sensors’ sensitivity and selectivity, allowing for accurate cyanidin detection and quantification. The electropolymerization procedure was used to create the electrode, and TEM, XRD, and Raman studies were used to confirm its structure. Using Differential Pulse Voltammetry (DPV), the electrochemical response of the MIP/MWCNTs/GCE sensor was investigated. With a detection limit of 0.14 mg/mL, the sensor showed a linear range of 0.5 mg/mL to 242 mg/mL, demonstrating good sensitivity and adaptability. Real samples (berry fruits, human urine, and tap water) were used to further validate the sensor’s performance, and the results revealed a strong correlation with the UPLC technique. The DPV approach showed excellent accuracy and suitability with low relative standard deviation values (less than 4.66%) and adequate recovery values (97.00–99.50%). Excellent selectivity, repeatability, and stability characterize the suggested MIP/MWCNTs/GCE sensor, which makes it a viable instrument for real-world uses in the food and environmental industries. This work advances the field by offering a more selective and sensitive way to identify cyanidin in berry fruits.