Purpose <p>Given that contact with the feces of dead triatomine bugs poses a risk of <i>Trypanosoma cruzi</i> infection, this study evaluated parasite viability and infectious potential in dead triatomines across various post-mortem times and temperatures.</p> Methods <p>Three groups of nine of 5th-instar <i>Triatoma pallidipennis</i> nymphs (<i>n</i> = 27) were euthanized via insecticide 30 days post-infection (Ninoa strain). Nymphs were maintained at room temperature (10 –23&#xa0;°C), refrigeration (4&#xa0;°C), and incubation (27&#xa0;°C). Fecal parasite concentration was assessed at 24&#xa0;h and at 2, 5, 7, 10, 15, 20, 25, and 30 days post-mortem. To evaluate infectivity, two CD1 mice were inoculated with intestinal contents from other two bugs kept for 30 days under each condition (<i>n</i> = 6), and parasitemia curves were generated.</p> Results <p>An increase in parasite counts in the feces of dead triatomines kept at room temperature was observed starting on day 15 post-mortem. This contrasted with the refrigeration and incubation groups, where parasite counts declined at days 5 and 7, respectively. Only mice inoculated with intestinal contents from the room temperature group showed positive parasitemia from day 20 onward.</p> Conclusions <p>Dead triatomines represent a potential infection source for <i>T. cruzi</i>, with storage temperature serving as a determining factor for parasite survival.</p>