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Background <p><i>Theileria</i> spp. are common tick-borne parasite in bovine animals, which can cause fever, anemia and emaciation after infection, imposing substantial economic losses to livestock.</p> Purpose <p>Ningxia is one of the most important dairy cattle production areas, but there was no report on the prevalence of <i>Theileria</i> spp. in dairy cattle till now.</p> Methods <p>The present study applied microscopic examination and PCR to investigate the infection condition of <i>Theileria</i> prevalence in 850 dairy cattle from three farms in Lingwu, Ningxia.</p> Results <p>A total of 140 (16.5%) animals were infected with <i>Theileria</i> spp. by blood smear examination. Further PCR-based sequencing examination targeting the 18&#xa0;S rRNA gene indicated 189 samples (22.2%) were positive with <i>Theileria</i> spp., including 143 samples with single peak and 46 with multiple peaks in sequencing. There existed significant differences on the positive rates of <i>Theileria</i> spp. in diary cattle across sampling sites, ages and seasons. Phylogenetic analysis showed all the 143 sequences with single peak were <i>Theileria orientalis</i>, yielding three genotypes with 96, 24 and 23 for Ikeda, Chitose and Buffeli, respectively.</p> Conclusion <p>Results of the present study indicates the existance of <i>T</i>. <i>orientalis</i> and frequent occurrence of Ikeda genotype for <i>T. orientalis</i> from dairy cattle in Lingwu, Ningxia, thus interventions are encouraged to reduce the potential damage caused by <i>Theileria</i> infection in this and those related regions to keep the safety of breeding industry.</p>

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Morphological Examination and Molecular Characterization of Theileria spp. From Dairy Cattle in Lingwu, Ningxia, China

  • 期刊论文

  • 发表日期：

    2026年3月24日

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  • Xinrui Hou,
  • Shinuo Liu,
  • Mengfan Xu,
  • Junke Song,
  • Xin Yang,
  • Guanghui Zhao,
  • Yingying Fan

摘要

Background

Theileria spp. are common tick-borne parasite in bovine animals, which can cause fever, anemia and emaciation after infection, imposing substantial economic losses to livestock.

Purpose

Ningxia is one of the most important dairy cattle production areas, but there was no report on the prevalence of Theileria spp. in dairy cattle till now.

Methods

The present study applied microscopic examination and PCR to investigate the infection condition of Theileria prevalence in 850 dairy cattle from three farms in Lingwu, Ningxia.

Results

A total of 140 (16.5%) animals were infected with Theileria spp. by blood smear examination. Further PCR-based sequencing examination targeting the 18 S rRNA gene indicated 189 samples (22.2%) were positive with Theileria spp., including 143 samples with single peak and 46 with multiple peaks in sequencing. There existed significant differences on the positive rates of Theileria spp. in diary cattle across sampling sites, ages and seasons. Phylogenetic analysis showed all the 143 sequences with single peak were Theileria orientalis, yielding three genotypes with 96, 24 and 23 for Ikeda, Chitose and Buffeli, respectively.

Conclusion

Results of the present study indicates the existance of T. orientalis and frequent occurrence of Ikeda genotype for T. orientalis from dairy cattle in Lingwu, Ningxia, thus interventions are encouraged to reduce the potential damage caused by Theileria infection in this and those related regions to keep the safety of breeding industry.