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<p>Gametocyte proteins are precursors of the oocyst wall proteins in <i>Eimeria</i> and play a crucial role in the development of immune-blocking vaccines and in reducing oocyst output. In this study, the gametocyte antigen gene En<i>gam59</i> from <i>E. necatrix</i> was selected to construct eukaryotic expression plasmid pVAX1-En<i>gam59</i>. This plasmid was then transferred into the attenuated <i>Salmonella</i> Enteritidis strain Z11Δ<i>rfbG</i> to construct the live vector vaccine Z11Δ<i>rfbG</i>(pVAX1-En<i>gam59</i>). The immune protective efficacy of the Z11Δ<i>rfbG</i>(pVAX1-En<i>gam59</i>) was subsequently evaluated through chicken immune protection trials. The results showed that pVAX1-En<i>gam59</i> was successfully expressed in Hek293t cells and was successfully transferred into Z11Δ<i>rfbG</i>. In vivo organs colonization tests showed that Z11Δ<i>rfbG</i>(pVAX1-En<i>gam59</i>) was more easily cleared by the host compared to Z11 wild-type strain. By day 14 post-infection, no bacteria were detected in the spleen or liver, and only a low amount of bacteria were found in the intestine. Chicken immune protection trials further revealed that immunization with Z11Δ<i>rfbG</i>(pVAX1-En<i>gam59</i>) stimulated lymphocyte proliferation and increased the levels of both anti-coccidial and anti-<i>Salmonella</i> antibodies in chickens. The anti-coccidial index (ACI) for chickens orally and intramuscularly administered Z11Δ<i>rfbG</i>(pVAX1-En<i>gam59</i>) was 157.7 and 157.9, respectively, indicating a moderate level of protection against coccidiosis. This study provides the foundation for the development of new live vector vaccines against coccidiosis.</p>

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Development and Evaluation of a Recombinant Attenuated Salmonella Enteritidis Vaccine Expressing the EnGAM59 Gametocyte Antigen of Eimeria necatrix for Coccidiosis Control

  • 期刊论文

  • 发表日期：

    2025年7月14日

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  • Dandan Liu,
  • Yongcui Feng,
  • Yu Zhang,
  • Feiyan Wang,
  • Xinyin Zhang,
  • Zhaofeng Hou,
  • Xilong Kang,
  • Jinjun Xu,
  • Zhiming Pan,
  • Jianping Tao

摘要

Gametocyte proteins are precursors of the oocyst wall proteins in Eimeria and play a crucial role in the development of immune-blocking vaccines and in reducing oocyst output. In this study, the gametocyte antigen gene Engam59 from E. necatrix was selected to construct eukaryotic expression plasmid pVAX1-Engam59. This plasmid was then transferred into the attenuated Salmonella Enteritidis strain Z11ΔrfbG to construct the live vector vaccine Z11ΔrfbG(pVAX1-Engam59). The immune protective efficacy of the Z11ΔrfbG(pVAX1-Engam59) was subsequently evaluated through chicken immune protection trials. The results showed that pVAX1-Engam59 was successfully expressed in Hek293t cells and was successfully transferred into Z11ΔrfbG. In vivo organs colonization tests showed that Z11ΔrfbG(pVAX1-Engam59) was more easily cleared by the host compared to Z11 wild-type strain. By day 14 post-infection, no bacteria were detected in the spleen or liver, and only a low amount of bacteria were found in the intestine. Chicken immune protection trials further revealed that immunization with Z11ΔrfbG(pVAX1-Engam59) stimulated lymphocyte proliferation and increased the levels of both anti-coccidial and anti-Salmonella antibodies in chickens. The anti-coccidial index (ACI) for chickens orally and intramuscularly administered Z11ΔrfbG(pVAX1-Engam59) was 157.7 and 157.9, respectively, indicating a moderate level of protection against coccidiosis. This study provides the foundation for the development of new live vector vaccines against coccidiosis.