Scholarlink

Purpose <p>Cystic echinococcosis is a parasitic zoonosis caused by the larval stage of <i>Echinococcus granulosus sensu lato</i>. Albendazole (ABZ) is the drug of choice, although its efficacy is variable. The present research aimed to assess the in vitro and in vivo efficacy of a <i>full-spectrum</i> extract of <i>Cannabis sativa</i> inflorescences against <i>E. granulosus sensu stricto</i> (<i>s.s.</i>).</p> Methods <p>Protoscoleces and cysts were incubated in vitro with the <i>C. sativa</i> extract, achieving final CBD concentrations of 1, 5, 10, and 50&#xa0;µg/ml. Viability was evaluated periodically. Structural and ultrastructural alterations were also recorded. For the clinical efficacy study, female CF-1 mice were infected. Six months later, mice were divided into groups (<i>n</i> = 10): (a) water control; (b) ABZ; (c) <i>C. sativa</i> extract, and (d) ABZ + <i>C. sativa</i> extract. Treatments were administered every 24&#xa0;h for 30 days. The efficacy of the treatments was evaluated according to the weight of the cysts collected and the ultrastructural alterations observed.</p> Results <p>The <i>C. sativa</i> extract caused a significant decrease in the viability of protoscoleces and cysts in vitro. The greatest effect was observed with 50&#xa0;µg/ml, which generated the reduction in protoscoleces viability to 0% between 6 and 24&#xa0;h post-incubation (pi) and the collapse of 92 ± 13% of the cysts after 24&#xa0;h pi. All the in vivo treatments reduced the weight of the cysts and caused ultrastructural alterations, especially the combination of ABZ + <i>C. sativa</i> extract.</p> Conclusion <p>We demonstrated the in vitro and in vivo efficacy of a <i>full-spectrum</i> extract of <i>C. sativa</i> inflorescences against <i>E. granulosus s.s</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

First Report of the Anti-Parasitic Effect of a Cannabis sativa full-spectrum Extract on Echinococcus granulosus sensu stricto

  • 期刊论文

  • 发表日期：

    2025年7月14日

查看全文
  • Florencia Gatti,
  • Clara María Albani,
  • Cristina Luján Ramírez,
  • Patricia Eugenia Pensel,
  • Adriana Andrea Albanese,
  • Giselle Magalí Fuentes,
  • Dalila Elisabet Orallo,
  • Diego Nutter,
  • María Celina Elissondo

摘要

Purpose

Cystic echinococcosis is a parasitic zoonosis caused by the larval stage of Echinococcus granulosus sensu lato. Albendazole (ABZ) is the drug of choice, although its efficacy is variable. The present research aimed to assess the in vitro and in vivo efficacy of a full-spectrum extract of Cannabis sativa inflorescences against E. granulosus sensu stricto (s.s.).

Methods

Protoscoleces and cysts were incubated in vitro with the C. sativa extract, achieving final CBD concentrations of 1, 5, 10, and 50 µg/ml. Viability was evaluated periodically. Structural and ultrastructural alterations were also recorded. For the clinical efficacy study, female CF-1 mice were infected. Six months later, mice were divided into groups (n = 10): (a) water control; (b) ABZ; (c) C. sativa extract, and (d) ABZ + C. sativa extract. Treatments were administered every 24 h for 30 days. The efficacy of the treatments was evaluated according to the weight of the cysts collected and the ultrastructural alterations observed.

Results

The C. sativa extract caused a significant decrease in the viability of protoscoleces and cysts in vitro. The greatest effect was observed with 50 µg/ml, which generated the reduction in protoscoleces viability to 0% between 6 and 24 h post-incubation (pi) and the collapse of 92 ± 13% of the cysts after 24 h pi. All the in vivo treatments reduced the weight of the cysts and caused ultrastructural alterations, especially the combination of ABZ + C. sativa extract.

Conclusion

We demonstrated the in vitro and in vivo efficacy of a full-spectrum extract of C. sativa inflorescences against E. granulosus s.s.