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Purpose <p>A previous study revealed that temnocephalids <i>Diceratocephala boschmai</i>, <i>Craspedella pedum</i>, and <i>Decadidymus</i> sp. were highly prevalent in <i>Cheraq quadricarinatus</i> in Melaka and Johor, Malaysia.</p> Methods <p>Herein, study was conducted to extract temnocephalids from <i>C. quadricarinatus</i> and identified using a molecular approach via two DNA markers: 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I (CO1). Thereafter, the population diversity of <i>D. boschmai</i> in Malaysia using the CO1 DNA marker was analysed to visualize the genetic associations among populations.</p> Results <p>The analysis of 16 variable (segregating) sites in the CO1 gene alignment of sequence from <i>D. boschmai</i> revealed a calculated haplotype diversity of 0.1266 among populations which indicates genetic variation within the studied groups. Additionally, the determination of nucleotide diversity (II) yielded a value of 0.0035, and Tajima's D neutrality test yielded a negative value for all populations, suggesting no significant departure from neutral evolution.</p> Conclusion <p>AMOVA analysis indicated that the genetic variation or polymorphism is primarily contributed within populations (95%) compared to between populations (4%).</p>

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Genetic and Population Diversity of Temnocephalids Associated with the Invasive Crayfish Cherax quadricarinatus in Malaysia

  • 期刊论文

  • 发表日期：

    2025年5月18日

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  • Norhan N. Azri-Shah,
  • Nor Asma Husna Yusoff,
  • Farizan Abdullah,
  • Ahmad Najmi Ishak,
  • Norainy Mohd Husin,
  • Hin Boo Wee,
  • John Brian Jones,
  • Muhammad Ikhwanuddin,
  • Siti Azizah Mohd Nor,
  • Marina Hassan

摘要

Purpose

A previous study revealed that temnocephalids Diceratocephala boschmai, Craspedella pedum, and Decadidymus sp. were highly prevalent in Cheraq quadricarinatus in Melaka and Johor, Malaysia.

Methods

Herein, study was conducted to extract temnocephalids from C. quadricarinatus and identified using a molecular approach via two DNA markers: 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I (CO1). Thereafter, the population diversity of D. boschmai in Malaysia using the CO1 DNA marker was analysed to visualize the genetic associations among populations.

Results

The analysis of 16 variable (segregating) sites in the CO1 gene alignment of sequence from D. boschmai revealed a calculated haplotype diversity of 0.1266 among populations which indicates genetic variation within the studied groups. Additionally, the determination of nucleotide diversity (II) yielded a value of 0.0035, and Tajima's D neutrality test yielded a negative value for all populations, suggesting no significant departure from neutral evolution.

Conclusion

AMOVA analysis indicated that the genetic variation or polymorphism is primarily contributed within populations (95%) compared to between populations (4%).