Scholarlink

Purpose <p><i>Paragonimus westermani</i> is the most common lung fluke infecting humans in Asia. This study aimed to molecularly characterize adult <i>P. westermani</i> from the south India (Kerala) using ITS-2, partial 28 S rDNA and partial <i>cox</i>1 gene sequences.</p> Methods <p>Adult flukes were recovered from the lungs of a tiger (<i>Panthera tigris</i>) (<i>n</i> = 10), a jungle cat (<i>Felis chaus</i>) (<i>n</i> = 6) and a domesticated dog (<i>Canis familiaris</i>) (<i>n</i> = 4). ITS-2, partial 28 SrDNA and partial <i>cox</i>1 gene sequences were amplified by polymerase chain reaction and a concatenated phylogenetic tree was constructed with these three marker sequences. Intra-species and inter-species genetic distances were calculated, and haplotype network analysis was performed using partial <i>cox</i>1 gene.</p> Results <p>Phylogenetic analysis based on the concatenated tree showed that Kerala isolates formed <i>P. westermani</i> complex with other isolates of <i>P. westermani</i> from India and from other countries. The Kerala isolates were genetically closer to metacercaria morphotype 2 of <i>P. westermani</i>. However, the genetic distance was higher among Indian isolates, indicating significant genetic variation among <i>P. westermani</i> within the complex. In addition, India also exhibited the highest number of haplotypes (<i>n</i> = 8).</p> Conclusions <p>Concatenated phylogenetic analysis of the Indian isolates of <i>P. westermani</i> show them to form <i>P. westermani</i> complex. The parasite exhibits high genetic distance with a large number of haplotypes. Further epidemiological studies are needed to understand its genetic variability. No sub-populations have been recognized in <i>P. westermani</i> based on the presented molecular data.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular Characterization of Paragonimus westermani from South India

  • 期刊论文

  • 发表日期：

    2025年5月16日

查看全文
  • Anisha Joy,
  • Anju Varghese,
  • Chundayil Kalarickal Deepa,
  • Karapparambu Gopalan Ajith Kumar,
  • Sebasteena Peekunnel Francis,
  • Aleena Iype,
  • Geethu Reghu,
  • Sreenidhi Nellikka Chalapron,
  • Anaswara Aravindhakshan,
  • George Chandy,
  • Pradeep Mampillikalam,
  • Hamza Palekkodan,
  • Reghu Ravindran

摘要

Purpose

Paragonimus westermani is the most common lung fluke infecting humans in Asia. This study aimed to molecularly characterize adult P. westermani from the south India (Kerala) using ITS-2, partial 28 S rDNA and partial cox1 gene sequences.

Methods

Adult flukes were recovered from the lungs of a tiger (Panthera tigris) (n = 10), a jungle cat (Felis chaus) (n = 6) and a domesticated dog (Canis familiaris) (n = 4). ITS-2, partial 28 SrDNA and partial cox1 gene sequences were amplified by polymerase chain reaction and a concatenated phylogenetic tree was constructed with these three marker sequences. Intra-species and inter-species genetic distances were calculated, and haplotype network analysis was performed using partial cox1 gene.

Results

Phylogenetic analysis based on the concatenated tree showed that Kerala isolates formed P. westermani complex with other isolates of P. westermani from India and from other countries. The Kerala isolates were genetically closer to metacercaria morphotype 2 of P. westermani. However, the genetic distance was higher among Indian isolates, indicating significant genetic variation among P. westermani within the complex. In addition, India also exhibited the highest number of haplotypes (n = 8).

Conclusions

Concatenated phylogenetic analysis of the Indian isolates of P. westermani show them to form P. westermani complex. The parasite exhibits high genetic distance with a large number of haplotypes. Further epidemiological studies are needed to understand its genetic variability. No sub-populations have been recognized in P. westermani based on the presented molecular data.