Background <p>Diabetic nephropathy (DN) is a leading cause of end-stage renal disease (ESRD) among individuals with diabetes, highlighting the urgent need for effective therapeutic strategies to combat this condition. Prior research has indicated that <i>T. spiralis</i> possesses hypoglycemic properties. In this investigation, we aimed to evaluate the efficacy of <i>T. spiralis</i> antigens, derived from both adult and larval forms, in treating diabetic nephropathy in alloxan-induced diabetic mice (AIDM).</p> Methods <p>A total of forty Swiss albino mice were allocated into four groups, each consisting of ten mice. Diabetes was induced in three of the groups using alloxan, while one group served as a control without diabetes. Two diabetic groups received treatment with either crude larva (CLA) antigen or adult worm antigen (AWA), while one group remained untreated. The study assessed various parameters, including fasting blood glucose levels, blood urea, serum creatinine, and serum albumin across all groups. Additionally, histopathological examinations of the kidneys were conducted. </p> Results <p>The results indicated that treatment with CLA or AWA antigens led to a significant reduction in blood glucose, serum creatinine, and blood urea levels, alongside an increase in serum albumin. Notably, the administration of AWA antigens resulted in substantial improvements in renal pathological changes induced by diabetes, as evidenced by hematoxylin and eosin staining and Masson trichrome staining, which also demonstrated a reduction in fibrosis. </p> Conclusions <p>The findings suggest that <i>T. spiralis</i> antigens may mitigate renal damage in diabetic mice by alleviating hyperglycemia-induced inflammation and oxidative stress, warranting further investigation into their potential role in preventing DN in diabetic patients.</p>