Scholarlink

Purpose <p>To investigate the seroprevalence and molecular characterization of <i>Anaplasma</i> parasites in mithun (<i>Bos frontalis</i>) and their tick vectors in Nagaland and Arunachal Pradesh, India using serological and molecular methods.</p> Methods <p>Mithun sera were screened for <i>Anaplasma</i> antibodies using a competitive ELISA targeting the MSP5 antigen. Blood samples and ticks collected from mithun were analyzed using PCR targeting the RNA polymerase subunit beta (<i>rpoB</i>) gene for <i>Anaplasma</i> species detection, followed by sequencing and phylogenetic analysis of positive samples.</p> Results <p>Serological testing detected <i>Anaplasma</i> antibodies in 11.90% (5/42) of tested mithun. PCR analysis of 358 mithun blood samples identified eight animals (2.23%) positive for <i>A. marginale</i>, with identity confirmed through sequencing and phylogenetic analysis. Among the collected ticks, <i>Anaplasma</i> was detected in one <i>R. microplus</i> specimen (1/340), while all <i>Amblyomma</i> spp. (<i>n</i> = 25) tested negative. Phylogenetic analysis of the <i>rpoB</i> gene sequences showed the mithun isolates clustering with <i>A. marginale</i> reference sequences from diverse regions.</p> Conclusion <p>This study provides the first documented evidence of <i>A. marginale</i> infection in mithun populations and associated ticks in Northeast India, supported by molecular, serological and phylogenetic analyses. The findings underscore the importance of multi-faceted diagnostic approaches, targeted vector control, and ongoing surveillance to address regional disease risks.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Report of Anaplasma marginale in Mithun (Bos frontalis) from North Eastern Hilly Region of India

  • 期刊论文

  • 发表日期：

    2025年2月13日

查看全文
  • Jayanta Kumar Chamuah,
  • Siju Susan Jacob,
  • Angughali Aheto Sumi,
  • Livika T. Awomi,
  • Lipenthang Ezung,
  • Mahak Singh,
  • S. Girish Patil

摘要

Purpose

To investigate the seroprevalence and molecular characterization of Anaplasma parasites in mithun (Bos frontalis) and their tick vectors in Nagaland and Arunachal Pradesh, India using serological and molecular methods.

Methods

Mithun sera were screened for Anaplasma antibodies using a competitive ELISA targeting the MSP5 antigen. Blood samples and ticks collected from mithun were analyzed using PCR targeting the RNA polymerase subunit beta (rpoB) gene for Anaplasma species detection, followed by sequencing and phylogenetic analysis of positive samples.

Results

Serological testing detected Anaplasma antibodies in 11.90% (5/42) of tested mithun. PCR analysis of 358 mithun blood samples identified eight animals (2.23%) positive for A. marginale, with identity confirmed through sequencing and phylogenetic analysis. Among the collected ticks, Anaplasma was detected in one R. microplus specimen (1/340), while all Amblyomma spp. (n = 25) tested negative. Phylogenetic analysis of the rpoB gene sequences showed the mithun isolates clustering with A. marginale reference sequences from diverse regions.

Conclusion

This study provides the first documented evidence of A. marginale infection in mithun populations and associated ticks in Northeast India, supported by molecular, serological and phylogenetic analyses. The findings underscore the importance of multi-faceted diagnostic approaches, targeted vector control, and ongoing surveillance to address regional disease risks.