<p>In the present work, the formation of self-assembled TiO₂ nanotubes (NTs) by anodic oxidation on Ti-13Nb-13Zr alloy and its effect on calcium phosphate phase enrichment on the alloy is reported. Anodization has been carried out in an electrolyte containing sulfuric acid and hydrofluoric acid. As-formed nanotubes have been characterized by x-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy dispersive x-ray (EDX) spectroscopy, Raman spectroscopy, and x-ray photoelectron spectroscopy (XPS). It has been noted that the native oxide present on the sample is substituted with self-assembled TiO₂ NTs arrays through the process of anodization. FESEM images show tubular morphology of nanotube arrays on anodized Ti-13Nb-13Zr alloy and white deposit corresponding to calcium phosphate is seen after immersing the sample in Hanks’ solution. The corrosion behavior of the anodized Ti-13Nb-13Zr alloy surface has been investigated with potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) studies in Hanks’ solution. The corrosion resistance of the anodized alloy sample is similar to that of the bare alloy sample. The effect of TiO₂ NTs on the enrichment of calcium phosphate over the anodized alloy sample has been investigated through immersion in Hanks’ solution for a duration of 7 days. FESEM images of immersed samples show that the anodized alloy surface exhibits higher deposits of calcium phosphate phase in comparison with the bare Ti-13Nb-13Zr alloy substrate. XPS analyses of immersed sample reveal a greater concentration of calcium, phosphorus, and oxygen in hydroxide/phosphate form on the anodized sample when compared to the bare alloy substrate.</p>