<p>Vanadium oxide quantum dots (VO_xQDs) with varying compositions exhibit unique optical properties that can be utilized in fluorescence sensing, bio-imaging, catalyzing, and other applications. It is necessary to regulate optical properties of VO_xQDs by exploring proper synthesis route. Herein, a solvothermal method was employed to synthesize VO_xQDs with the particle size of 4.3 ± 0.8 nm, quantum yield of 4.97% and lattice spacing of about 0.184 nm, using vanadium trioxide (V₂O₃) as the precursor in this work. In addition, as-prepared VO_xQDs exhibited extensive UV absorption at 301 nm and contained V³⁺, V⁴⁺and V⁵⁺. This mixed valence system resulted in the presence of multiple fluorophores with distinct chemical structures, which generated excitation peaks at both 321 and 341&#xa0;nm, along with fluorescence emission at both 399 and 423&#xa0;nm when excited at 341&#xa0;nm. This special characteristic can be leveraged for the development of multiple fluorescence probes. Therefore, this work provided additional options for the synthesis of VO_xQDs with special photoluminescence (PL) properties, which may have potential applications in anti-counterfeiting packaging. Furthermore, specialized excitation wavelengths could activate the emission peaks, and enable rapid identification of product authenticity.</p>