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<p>The increasing global prevalence of diabetes necessitates the development of accurate, reliable, and accessible glucose monitoring devices, particularly for point-of-care testing. In this study, we developed an electrochemical glucose biosensor using electrospun polyvinyl alcohol (PVA) nanofibers. Among three immobilization strategies, dip-coating glucose oxidase (GOx) exhibits superior electrochemical reversibility and a lower operating potential compared to drop-casting and electrospinning techniques. By integrating reduced graphene oxide (rGO) to enhance the conductivity of the polymer matrix, the resulting dip-GOx/rGO/PVA biosensor demonstrated significantly enhanced electron transfer kinetics (<InlineEquation ID="IEq1"> <EquationSource Format="TEX">\(k_{{\text{s}}} = 0.36\,{\text{s}}^{ - 1}\)</EquationSource> <EquationSource Format="MATHML"><math> <mrow> <msub> <mi>k</mi> <mtext>s</mtext> </msub> <mo>=</mo> <mn>0.36</mn> <mspace width="0.166667em" /> <msup> <mrow> <mtext>s</mtext> </mrow> <mrow> <mo>-</mo> <mn>1</mn> </mrow> </msup> </mrow> </math></EquationSource> </InlineEquation>) and an increase in sensitivity from 35.41 µA&#xa0;mM<sup>−1</sup>&#xa0;cm<sup>−2</sup> to 51.36 µA&#xa0;mM<sup>−1</sup>&#xa0;cm<sup>−2</sup>. The sensor maintained a linear response range of 3–9&#xa0;mM with a limit of detection of 1.23&#xa0;mM, covering the critical physiological glucose levels. Although less sensitive than noble-metal sensors, this platform is cost-effective, eco-friendly, and highly biocompatible, making it a sustainable candidate for disposable biosensors.</p> Graphical Abstract <p></p>

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Electrospun rGO/PVA Nanofibrous Biosensors: Impact of Enzyme Immobilization on Electrochemical Performance

  • 期刊论文

  • 发表日期：

    2026年7月21日

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  • Hoai Phuong Vo,
  • Minh Phong Nguyen,
  • Minh Doan Nguyen,
  • Thi Dao Nguyen,
  • Hai Dang Nguyen,
  • Ngoc Thao Pham,
  • Duc San Nguyen,
  • Thi Thu Thuy Can,
  • Van Quang Nguyen,
  • Duy Tho Pham,
  • The-Long Phan,
  • Tuan Canh Nguyen

摘要

The increasing global prevalence of diabetes necessitates the development of accurate, reliable, and accessible glucose monitoring devices, particularly for point-of-care testing. In this study, we developed an electrochemical glucose biosensor using electrospun polyvinyl alcohol (PVA) nanofibers. Among three immobilization strategies, dip-coating glucose oxidase (GOx) exhibits superior electrochemical reversibility and a lower operating potential compared to drop-casting and electrospinning techniques. By integrating reduced graphene oxide (rGO) to enhance the conductivity of the polymer matrix, the resulting dip-GOx/rGO/PVA biosensor demonstrated significantly enhanced electron transfer kinetics ( \(k_{{\text{s}}} = 0.36\,{\text{s}}^{ - 1}\) k s = 0.36 s - 1 ) and an increase in sensitivity from 35.41 µA mM−1 cm−2 to 51.36 µA mM−1 cm−2. The sensor maintained a linear response range of 3–9 mM with a limit of detection of 1.23 mM, covering the critical physiological glucose levels. Although less sensitive than noble-metal sensors, this platform is cost-effective, eco-friendly, and highly biocompatible, making it a sustainable candidate for disposable biosensors.

Graphical Abstract