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<p><i>Staphylococcus</i> <i>aureus</i> (<i>S.</i> <i>aureus</i>) is recognized for causing various diseases and for the emergence of drug-resistant strains frequently encountered in healthcare settings. Toxic shock syndrome caused by this bacterium is one of the most lethal conditions, potentially leading to death within hours if not swiftly recognized. This research aims to create an electrochemical sensor by fabricating the surface of a bare pencil graphite electrode through electrodeposition or electropolymerization techniques using copper nanoparticles and pyrrole−2,5-dicarboxylic acid. This sensor is designed to be optimized for the detection of <i>S.</i> <i>aureus</i> through cyclic voltammetry and square wave voltammetry techniques. The modified nanoscale surfaces were examined using scanning electron microscopy. The fabricated electrode demonstrated an electron transfer coefficient of <i>α</i>&#xa0;=&#xa0;0.83, a logarithmic value of the kinetic constant of log Ks&#xa0;=&#xa0;3.09, and a concentration of the active surface layer measured at Г&#xa0;=&#xa0;2.38&#xa0;×&#xa0;10<sup>−5</sup>&#xa0;mole&#xa0;cm<sup>−2</sup>. Under the optimized chemical and instrumental parameters, <i>S.</i> <i>aureus</i> was quantified utilizing the modified electrode across a concentration range of 400–5600 colonies per volume (CFU/mL), achieving a detection limit of 133&#xa0;CFU/mL. The sensor not only boasts simplicity and affordability but also provides advantages, including a low detection limit, an appropriate linear range, elevated sensitivity, and outstanding reproducibility. This newly developed electrochemical sensor has the potential to function as an accurate and reliable instrument for the detection of <i>S.</i> <i>aureus</i> in human blood serum samples, effectively addressing significant challenges.</p> Graphical Abstract <p></p>

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An Innovative Electrochemical Sensor Designed as PGE/CuNPs/Poly-PDC for the Identification and Quantification of Staphylococcus aureus

  • 期刊论文

  • 发表日期：

    2025年5月16日

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  • Zahra Ghorbani,
  • Seyed Mohammad Mahdi Hamdi,
  • Fariba Lahoorpour,
  • Arash Babakhanian,
  • Kumarss Amini

摘要

Staphylococcus aureus (S. aureus) is recognized for causing various diseases and for the emergence of drug-resistant strains frequently encountered in healthcare settings. Toxic shock syndrome caused by this bacterium is one of the most lethal conditions, potentially leading to death within hours if not swiftly recognized. This research aims to create an electrochemical sensor by fabricating the surface of a bare pencil graphite electrode through electrodeposition or electropolymerization techniques using copper nanoparticles and pyrrole−2,5-dicarboxylic acid. This sensor is designed to be optimized for the detection of S. aureus through cyclic voltammetry and square wave voltammetry techniques. The modified nanoscale surfaces were examined using scanning electron microscopy. The fabricated electrode demonstrated an electron transfer coefficient of α = 0.83, a logarithmic value of the kinetic constant of log Ks = 3.09, and a concentration of the active surface layer measured at Г = 2.38 × 10−5 mole cm−2. Under the optimized chemical and instrumental parameters, S. aureus was quantified utilizing the modified electrode across a concentration range of 400–5600 colonies per volume (CFU/mL), achieving a detection limit of 133 CFU/mL. The sensor not only boasts simplicity and affordability but also provides advantages, including a low detection limit, an appropriate linear range, elevated sensitivity, and outstanding reproducibility. This newly developed electrochemical sensor has the potential to function as an accurate and reliable instrument for the detection of S. aureus in human blood serum samples, effectively addressing significant challenges.

Graphical Abstract