Polymeric Biosensor Development for Electrochemical Analysis of Tartrazine and Methyl Orange

In the current work, an electrochemical electrode was designed for the detection of tartrazine (TT) with good selectivity and sensitivity. A tyrosine (TY)-modified carbon paste electrode P(TY)MCPE was used for the detection of TT using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The surface characteristics of both the bare carbon paste electrode (BCPE) and P(TY)MCPE were studied by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The optimum conditions for the study of TT were obtained by studying the scan rate, effect of pH, accumulation potential, time, and variation of concentration. The modified electrode exhibited a good response in comparison with the unmodified electrode under ideal conditions. The P(TY)MCPE displayed good electrochemical performance for concentration of TT in the linear range of 2.0–80.0 μM with a limit of quantification (LOQ) of 1.676 μM and limit of detection (LOD) of 0.502 μM. The fabricated electrode exhibited good reproducibility, repeatability, and stability. In the presence of methyl orange (MO) and other interferents, the modified P(TY)MCPE exhibited good selectivity for TT. The P(TY)MCPE electrode was successfully applied for the determination of TT in a real sample with a satisfactory recovery rate.