Scholarlink

Objective <p>To investigate the anti-colorectal cancer (CRC) activity and its potential molecular mechanism of euscaphic acid (EA), a triterpene component from <i>Rosa roxburghii</i> Tratt (RRT), <i>in vitro</i> and <i>in vivo</i>.</p> Methods <p>EA in RRT was isolated and analyzed for pharmacophore-based virtual screening, and enrichment analysis was performed. HCT116 and CT26 cells were used for <i>in vitro</i> experiments. Meanwhile, we constructed a CT26 syngeneic tumor BALB/c mice model and performed <i>in vivo</i> experiments. Finally, the mitochondrial apoptosis and reactive oxygen species (ROS)/mitogen-activated protein kinase (MAPK) signaling pathway-related proteins were detected by Western blot.</p> Results <p>EA could inhibit the viability of HCT116 and CT26 cell lines, suppress the biological behaviors including proliferation, migration, and invasion, and induce apoptosis <i>in vitro</i> (<i>P</i>&lt;0.05 or <i>P</i>&lt;0.01). Meanwhile, <i>in vivo</i> experiments showed that EA significantly inhibited the growth of CRC graft tumors (<i>P</i>&lt;0.05 or <i>P</i>&lt;0.01). EA could decrease mitochondrial membrane potential and increase caspase-3/9 activities (<i>P</i>&lt;0.05 or <i>P</i>&lt;0.01). Furthermore, EA promoted the cellular ROS accumulation accompanied by activation of the MAPK signaling pathway, and interestingly, the phosphorylation level of extracellular regulated protein kinases (ERK) was higher (<i>P</i>&lt;0.05 or <i>P</i>&lt;0.01). In addition, the above process could be reversed by the free radical scavenger N-acetylcysteine (<i>P</i>&lt;0.05 or <i>P</i>&lt;0.01).</p> Conclusions <p>EA could inhibit the growth of CRC cell lines both <i>in vitro</i> and <i>in vivo</i>, and activate the mitochondrial pathway through the ROS/MAPK signaling pathway to induce apoptosis in CRC cell lines. This supports the antitumor potential of triterpene acids from RRT.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Euscaphic Acid from Rosa roxburghii Tratt Exerts Anti-colorectal Cancer Activity by Inducing Mitochondrial Apoptosis through ROS/MAPK Pathway

  • 期刊论文

  • 发表日期：

    2025年10月20日

查看全文
  • Mao-yu Zhang,
  • Bo-wen Zhai,
  • Han Huang,
  • Heng Zhao,
  • Yu-jie Fu

摘要

Objective

To investigate the anti-colorectal cancer (CRC) activity and its potential molecular mechanism of euscaphic acid (EA), a triterpene component from Rosa roxburghii Tratt (RRT), in vitro and in vivo.

Methods

EA in RRT was isolated and analyzed for pharmacophore-based virtual screening, and enrichment analysis was performed. HCT116 and CT26 cells were used for in vitro experiments. Meanwhile, we constructed a CT26 syngeneic tumor BALB/c mice model and performed in vivo experiments. Finally, the mitochondrial apoptosis and reactive oxygen species (ROS)/mitogen-activated protein kinase (MAPK) signaling pathway-related proteins were detected by Western blot.

Results

EA could inhibit the viability of HCT116 and CT26 cell lines, suppress the biological behaviors including proliferation, migration, and invasion, and induce apoptosis in vitro (P<0.05 or P<0.01). Meanwhile, in vivo experiments showed that EA significantly inhibited the growth of CRC graft tumors (P<0.05 or P<0.01). EA could decrease mitochondrial membrane potential and increase caspase-3/9 activities (P<0.05 or P<0.01). Furthermore, EA promoted the cellular ROS accumulation accompanied by activation of the MAPK signaling pathway, and interestingly, the phosphorylation level of extracellular regulated protein kinases (ERK) was higher (P<0.05 or P<0.01). In addition, the above process could be reversed by the free radical scavenger N-acetylcysteine (P<0.05 or P<0.01).

Conclusions

EA could inhibit the growth of CRC cell lines both in vitro and in vivo, and activate the mitochondrial pathway through the ROS/MAPK signaling pathway to induce apoptosis in CRC cell lines. This supports the antitumor potential of triterpene acids from RRT.