Development of an in vitro micropropagation protocol for the sustainable conservation of the local endemic Lilium akkusianum
摘要
Bulbous ornamental plants are of great importance in plant biotechnology and conservation biology due to their ecological functions and high economic value. This study was carried out to evaluate the hormone, culture medium, and light factors affecting bulblet formation in Lilium akkusianum, a locally endemic and endangered plant, under in vitro culture conditions. Leaf and root explants were examined in two culture media (MS and B5), different 2,4-D (0.1, 1.0, and 2.0 mg L⁻1) and kinetin (0.1, 1.0 mg L⁻1) doses, and two different climate conditions (25 ± 2 °C temperature and 15 d of continuous darkness, 25 ± 2 °C temperature, 16/8 h light/dark, and 3.000 lx light intensity). Results showed that MS medium provided a higher regeneration capacity compared to B5 medium. Leaf explants formed more bulblets than root explants. The highest bulblet formation was obtained from leaf explants with the combination of MS containing 2.0 mg L−1 2,4-D and 1.0 mg L−1 kinetin (8.33 pieces) under dark conditions. In root explants, MS with 1.0 mg L−1 2,4-D and 0.1 mg L−1 kinetin medium and dark conditions (13.3 pieces) were the prominent combination. These results suggested that an auxin-cytokinin balance plays a critical role in organogenesis and that light conditions significantly affect the embryogenic response. This study provided a scientific basis for the development of an effective micropropagation protocol for this local endemic species and contributed to the conservation of the species and to the increase of its production potential as an ornamental plant.