In vitro culture studies and the effect of ZnO and CuO nanoparticles on total phenolic content expression in psoralea corylifolia
摘要
In vitro cultures have the potential to serve as bio-factories for the production of secondary metabolites when stimulated by various stress factors. This study aimed to establish tissue culture techniques and to explore the use of nanoparticles as elicitors to enhance the production of total phenolic content (TPC) in Psoralea corylifolia. Healthy callus induction from nodal explants was achieved using Murashige and Skoog (MS) basal medium supplemented with 18.09 µM 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.86 µM kinetin (KIN). Zinc oxide (ZnO) and copper oxide (CuO) nanoparticles (NPs) were synthesized and characterized using scanning electron microscopy (SEM) and, subsequently, were evaluated for their ability to enhance biomass accumulation and TPC production. The optimal TPC accumulation for ZnO-NPs was 10.0 mg L−1, combined with 6.66 µM of 6-benzylaminopurine (BAP), and yielding 3.51 mg GAE g⁻1 dry weight (DW) on 14th d. In contrast, CuO-NPs achieved the highest TPC of 5.03 mg GAE g⁻1 DW at a concentration of 50 mg L−1 on 7th d. These results highlighted the potential of metal oxide nanoparticles as effective elicitors for sustainably enhancing phenolic compound production in P. corylifolia cultures. This innovative approach offers a promising strategy for conserving this endangered species while expanding its applications in biotechnological industries.