Tailoring traits in agricultural and horticultural crops using CRISPR-Cas9 for enhanced abiotic, biotic, and consumer-favored attributes
摘要
CRISPR-Cas9 genome editing technology has modernized plant science by enabling precise modifications of genetic material to develop crops with tailored traits. In agricultural and horticultural systems, CRISPR-Cas9 offers immense potential to address critical challenges, including abiotic and biotic stresses, while aligning crop attributes with consumer demands. Abiotic stresses, such as drought, salinity, and extreme temperatures, as well as biotic factors, like pests and pathogens, significantly affect crop yield and quality. Through targeted gene editing, CRISPR-Cas9 facilitates the development of resilient crop varieties capable of thriving under adverse environmental conditions. Interestingly, CRISPR-Cas9 has been instrumental in enhancing consumer-favored traits, such as improved nutritional profiles and prolonged shelf-life properties. This technology allows the precise insertion, deletion, or alteration of specific genes, thereby evading the limitations of conventional breeding and transgenic approaches. The application of CRISPR-Cas9 is poised to address the dual challenges of food security and sustainability by delivering crops that are not only productive and resilient but also meet market preferences. Despite its transformative potential, public acceptance and regulatory frameworks for CRISPR-edited crops remain areas of active debate. Policymakers and stakeholders must navigate these challenges to unlock the full potential of this technology. As research progresses, CRISPR-Cas9 is expected to be a transformative tool in advancing agriculture and horticulture, driving innovation to meet the growing demands of the changing world. This review focuses on the recent integration of CRISPR-Cas9 in tailoring traits for enhanced resistance to abiotic and biotic factors, as well as consumer-preferred characteristics.