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<p><i>Andrographis paniculata</i> (Burm.f.) Nees, a medicinal plant renowned for its bioactive compound andrographolide, holds significant pharmacological importance. Colchiploidy, a process involving chromosome set duplication, presents an innovative approach to enhancing secondary metabolite production. This study reports the successful <i>in vitro</i> micropropagation of nodal explants from diploid and colchiploid <i>A. paniculata</i> under identical culture conditions. Ploidy levels were confirmed <i>via</i> flow cytometry. Optimal shoot regeneration was achieved on half-strength Murashige and Skoog (MS) medium supplemented with 4.44&#xa0;µM BAP, while root induction was enhanced using half-strength MS medium containing 2.5&#xa0;µM IBA. The incorporation of 100.0&#xa0;mg L⁻<sup>1</sup> activated charcoal significantly improved shoot elongation and root development. Notably, HPLC analysis revealed a 150-fold increase in andrographolide content in colchiploid plants compared to their diploid counterparts. This is the first study to document the <i>in vitro</i> regeneration of colchiploid <i>A. paniculata</i> and quantify andrographolide from <i>in vitro</i>–derived colchiploid plants. These findings address a critical gap in the literature and demonstrate the immense potential of colchiploidy as a strategy for enhancing secondary metabolite production in medicinal plants. This pioneering approach offers new avenues for sustainable biotechnological applications in plant-based pharmaceuticals.</p>

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Maximizing andrographolide production through optimized in vitro micropropagation of colchiploid Andrographis paniculata (Burm.f.) Nees

  • 期刊论文

  • 发表日期：

    2025年11月24日

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  • Lekshmi Priya,
  • P. R. Unnikrishna Pillai

摘要

Andrographis paniculata (Burm.f.) Nees, a medicinal plant renowned for its bioactive compound andrographolide, holds significant pharmacological importance. Colchiploidy, a process involving chromosome set duplication, presents an innovative approach to enhancing secondary metabolite production. This study reports the successful in vitro micropropagation of nodal explants from diploid and colchiploid A. paniculata under identical culture conditions. Ploidy levels were confirmed via flow cytometry. Optimal shoot regeneration was achieved on half-strength Murashige and Skoog (MS) medium supplemented with 4.44 µM BAP, while root induction was enhanced using half-strength MS medium containing 2.5 µM IBA. The incorporation of 100.0 mg L⁻1 activated charcoal significantly improved shoot elongation and root development. Notably, HPLC analysis revealed a 150-fold increase in andrographolide content in colchiploid plants compared to their diploid counterparts. This is the first study to document the in vitro regeneration of colchiploid A. paniculata and quantify andrographolide from in vitro–derived colchiploid plants. These findings address a critical gap in the literature and demonstrate the immense potential of colchiploidy as a strategy for enhancing secondary metabolite production in medicinal plants. This pioneering approach offers new avenues for sustainable biotechnological applications in plant-based pharmaceuticals.