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<p>Salinity stress limits plant growth and productivity around the world. In the present work, we investigated the relation of the reactive oxygen species (apROS) and antioxidant system in the poplar apoplast when exposing NL895 poplar plantlets to 50 and 100&#xa0;mM NaCl. In addition, we analyzed the responses of apoplastic proteins (apPRs) to salt stress by 2D-electrophoresis and mass spectrometry. The results showed that 50–100&#xa0;mM NaCl treatment enhanced the activities of apPOD, apAPX, apGR, and apDHAR, and increased the levels of apAsA and apGSH. Ratios of apAsA/apDHA and apGSH/apGSSG (cellular redox potential) also elevated under salinity (100&#xa0;mM NaCl). Meanwhile, the levels of apH<sub>2</sub>O<sub>2</sub> increased significantly under salinity. These indicated that the AsA-GSH reduction/oxidation (R/O) state plays an important role in the apoplast response to salinity stress. The thicker cell wall and more fillings in vascular bundles in NaCl-treated plantlets were observed by scanning electron microscopy. The apPOD activity and apH<sub>2</sub>O<sub>2</sub> level were related to cell wall modification of ‘NL895’ poplar. Between control and 100&#xa0;mM NaCl-treated leaves, 23 apPRs were identified (∣fold change∣ &gt; 2.0-fold). These apPRs were mainly enriched in response and defense proteins to salt stress and ferritin metabolism. Comparative proteomic analysis revealed that these apPRs were related to the antioxidant system, heme-containing synthesis, and protein folding. Hsp70 was an important apoplastic protein and was involved in response to salinity stress in ‘NL895’&#xa0;poplar apoplast.</p>

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Apoplastic proteome and antioxidant system responses to in vitro salinity stress in ‘NL895’ poplar plantlets

  • 期刊论文

  • 发表日期：

    2025年10月1日

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  • Tingting Feng,
  • Jiaxin Zhuge,
  • Ying Chen,
  • Jingjing Di,
  • Li Yue,
  • Zihan Wei

摘要

Salinity stress limits plant growth and productivity around the world. In the present work, we investigated the relation of the reactive oxygen species (apROS) and antioxidant system in the poplar apoplast when exposing NL895 poplar plantlets to 50 and 100 mM NaCl. In addition, we analyzed the responses of apoplastic proteins (apPRs) to salt stress by 2D-electrophoresis and mass spectrometry. The results showed that 50–100 mM NaCl treatment enhanced the activities of apPOD, apAPX, apGR, and apDHAR, and increased the levels of apAsA and apGSH. Ratios of apAsA/apDHA and apGSH/apGSSG (cellular redox potential) also elevated under salinity (100 mM NaCl). Meanwhile, the levels of apH2O2 increased significantly under salinity. These indicated that the AsA-GSH reduction/oxidation (R/O) state plays an important role in the apoplast response to salinity stress. The thicker cell wall and more fillings in vascular bundles in NaCl-treated plantlets were observed by scanning electron microscopy. The apPOD activity and apH2O2 level were related to cell wall modification of ‘NL895’ poplar. Between control and 100 mM NaCl-treated leaves, 23 apPRs were identified (∣fold change∣ > 2.0-fold). These apPRs were mainly enriched in response and defense proteins to salt stress and ferritin metabolism. Comparative proteomic analysis revealed that these apPRs were related to the antioxidant system, heme-containing synthesis, and protein folding. Hsp70 was an important apoplastic protein and was involved in response to salinity stress in ‘NL895’ poplar apoplast.