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<p><i>Mammillaria</i><i> candida</i> Scheidweiler is a species considered threatened according to the NOM-059-SEMARNAT-2010. With the purpose of rescuing this species and massively propagating it for its marketing and possibly the reintroduction to its natural habitat, the present authors studied and established the bases for <i>in vitro</i> regeneration of complete plants acclimated to <i>ex vitro</i> conditions through the implementation of a micropropagation protocol based on the induction of direct morphogenetic responses. In the induction stage, the effect of different cytokinins, including benzylaminopurine (BAP), kinetin (KN), and N6-(2-isopentenyl) adenine (2ip), alone and in combination with naphthaleneacetic acid (NAA) was evaluated, and the best treatments were re-evaluated during the proliferation stage.&#xa0;Kinetin at 65.04&#xa0;µM resulted in the optimal induction and proliferation of shoots, with a mean of 18.7 shoots per explant. For rhizogenesis, four concentrations of indolebutyric acid (IBA) were evaluated, in addition to the aggregation of different concentrations of calcium (CaCl<sub>2</sub>) to the Murashige and Skoog (MS) basal medium at 100% and 50% of salts. The best treatment was 4.0&#xa0;g L<sup>−1</sup> CaCl<sub>2</sub> and 1.0&#xa0;mg L<sup>−1</sup> IBA in full strength MS medium with a total of 10.8 roots per explant and 18.9&#xa0;mm of their length on average. For the acclimatization of the plants, the effect of commercial mycorrhizae inoculated to the substrate was evaluated by varying the concentration of phosphorus in fertilization, where the survival and growth of the plants after 180&#xa0;days (d) of acclimatization was determined. The best treatment was the mycorrhized plants with a phosphorus variation of 66&#xa0;ppm (20.5&#xa0;mm in diameter and 9.6&#xa0;mm in height on average), in addition to 100% survival of the specimens in <i>ex vitro</i> conditions.</p>

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In vitro regeneration of Mammillaria candida Scheidweiler (Cactaceae)

  • 期刊论文

  • 发表日期：

    2025年10月10日

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  • Abraham A. Arellano-Perusquía,
  • Andrés Cruz-Hernandez,
  • Sinue I. Morales-Alonso

摘要

Mammillaria candida Scheidweiler is a species considered threatened according to the NOM-059-SEMARNAT-2010. With the purpose of rescuing this species and massively propagating it for its marketing and possibly the reintroduction to its natural habitat, the present authors studied and established the bases for in vitro regeneration of complete plants acclimated to ex vitro conditions through the implementation of a micropropagation protocol based on the induction of direct morphogenetic responses. In the induction stage, the effect of different cytokinins, including benzylaminopurine (BAP), kinetin (KN), and N6-(2-isopentenyl) adenine (2ip), alone and in combination with naphthaleneacetic acid (NAA) was evaluated, and the best treatments were re-evaluated during the proliferation stage. Kinetin at 65.04 µM resulted in the optimal induction and proliferation of shoots, with a mean of 18.7 shoots per explant. For rhizogenesis, four concentrations of indolebutyric acid (IBA) were evaluated, in addition to the aggregation of different concentrations of calcium (CaCl2) to the Murashige and Skoog (MS) basal medium at 100% and 50% of salts. The best treatment was 4.0 g L−1 CaCl2 and 1.0 mg L−1 IBA in full strength MS medium with a total of 10.8 roots per explant and 18.9 mm of their length on average. For the acclimatization of the plants, the effect of commercial mycorrhizae inoculated to the substrate was evaluated by varying the concentration of phosphorus in fertilization, where the survival and growth of the plants after 180 days (d) of acclimatization was determined. The best treatment was the mycorrhized plants with a phosphorus variation of 66 ppm (20.5 mm in diameter and 9.6 mm in height on average), in addition to 100% survival of the specimens in ex vitro conditions.