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<p>This study aimed to develop an efficient <i>in vitro</i> micropropagation protocol for <i>Dyckia ibiramensis</i> Reitz, an endangered and endemic bromeliad species from the Brazilian Atlantic Forest. The following were evaluated in this study: (i) the effects of Murashige and Skoog (MS) salt concentrations, temperature regimes, and photoperiods on slow growth storage; (ii) varying concentrations of 6-benzylaminopurine (BAP) or meta-topolin (mT) combined with naphthaleneacetic acid (NAA) during shoot multiplication; (iii) different concentrations of indole-3-butyric acid (IBA), with or without activated charcoal, for rooting; and (iv) substrate compositions involving commercial soil, grit, and vermiculite during acclimatization. Optimal slow growth conditions were achieved with half-strength MS medium under a 12-h photoperiod at 10°C. The highest multiplication rate occurred with 2.0&#xa0;µM mT plus 1.0&#xa0;µM NAA. Rooting was most effective following a 15-d exposure to 5.0&#xa0;µM IBA. For acclimatization, the best plantlet survival was observed with commercial substrate alone or combined with grit and vermiculite. A key strength of this protocol is the use of seed-derived explants, which preserve the genetic variability of natural populations—an essential feature for the long-term conservation of <i>D. ibiramensis</i>.</p>

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In vitro propagation and slow growth storage of the endangered Brazilian bromeliad Dyckia ibiramensis Reitz

  • 期刊论文

  • 发表日期：

    2025年10月30日

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  • Iasmin Tassi Grott,
  • Hugo Pacheco de Freitas Fraga,
  • Miguel Pedro Guerra,
  • Ricardo Bittencourt

摘要

This study aimed to develop an efficient in vitro micropropagation protocol for Dyckia ibiramensis Reitz, an endangered and endemic bromeliad species from the Brazilian Atlantic Forest. The following were evaluated in this study: (i) the effects of Murashige and Skoog (MS) salt concentrations, temperature regimes, and photoperiods on slow growth storage; (ii) varying concentrations of 6-benzylaminopurine (BAP) or meta-topolin (mT) combined with naphthaleneacetic acid (NAA) during shoot multiplication; (iii) different concentrations of indole-3-butyric acid (IBA), with or without activated charcoal, for rooting; and (iv) substrate compositions involving commercial soil, grit, and vermiculite during acclimatization. Optimal slow growth conditions were achieved with half-strength MS medium under a 12-h photoperiod at 10°C. The highest multiplication rate occurred with 2.0 µM mT plus 1.0 µM NAA. Rooting was most effective following a 15-d exposure to 5.0 µM IBA. For acclimatization, the best plantlet survival was observed with commercial substrate alone or combined with grit and vermiculite. A key strength of this protocol is the use of seed-derived explants, which preserve the genetic variability of natural populations—an essential feature for the long-term conservation of D. ibiramensis.