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<p><i>Salvia sclarea</i> L. is an economically potent aromatic plant well known for its therapeutic and nutraceutical potential. The present experiment aimed to develop a rapid, simple, and replicable regeneration system with increased regeneration efficiency, using hypocotyls as explants on optimal medium and culture conditions. Six-day-old hypocotyl explants produced the highest multiple shoots on an MS medium augmented with 3.63&#xa0;µM TDZ, resulting in an average of 34.33 ± 0.33 shoots per explant, with a maximum length of 9.23 ± 0.17&#xa0;cm. The rooting frequency was 100%, and the highest root induction, 5.64 ± 2.64 roots per explant of 9.25 ± 1.52&#xa0;cm length, was attained on ½ MS basal medium. Successfully rooted plantlets were acclimatized to <i>ex vitro</i> conditions, with a survival rate exceeding 90%. Further, molecular and biochemical analyses confirmed the homogeneity/monomorphic nature of regenerated and wild plants. Biochemical analysis was done, followed by molecular confirmation of the regenerated plants vs. mother plants. The mother and <i>in vitro</i> regenerated plantlets contain diverse biochemical compounds with important pharmacological properties. Furthermore, the biochemical composition of the two is substantially comparable, indicating their metabolic similarities. The genetic similarity between both plants was confirmed by the presence of monomorphic bands generated using the Start Codon Targeted (SCoT) and Inter Simple Sequence Repeat (ISSR) markers. Morphological variation was studied using scanning electron microscopy (SEM) analysis. SEM observations revealed the uniform arrangements of trichomes and stomata during the <i>in vitro</i> regeneration.</p>

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Potential use of TDZ to improve regeneration efficiency in Salvia sclarea L.: a molecular and biochemical assessment for genetic purity

  • 期刊论文

  • 发表日期：

    2025年9月22日

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  • Shafali Bhasin,
  • Vanila Sharma,
  • Yadunandan Sen,
  • Srinivas Kota,
  • Dhiraj Vyas

摘要

Salvia sclarea L. is an economically potent aromatic plant well known for its therapeutic and nutraceutical potential. The present experiment aimed to develop a rapid, simple, and replicable regeneration system with increased regeneration efficiency, using hypocotyls as explants on optimal medium and culture conditions. Six-day-old hypocotyl explants produced the highest multiple shoots on an MS medium augmented with 3.63 µM TDZ, resulting in an average of 34.33 ± 0.33 shoots per explant, with a maximum length of 9.23 ± 0.17 cm. The rooting frequency was 100%, and the highest root induction, 5.64 ± 2.64 roots per explant of 9.25 ± 1.52 cm length, was attained on ½ MS basal medium. Successfully rooted plantlets were acclimatized to ex vitro conditions, with a survival rate exceeding 90%. Further, molecular and biochemical analyses confirmed the homogeneity/monomorphic nature of regenerated and wild plants. Biochemical analysis was done, followed by molecular confirmation of the regenerated plants vs. mother plants. The mother and in vitro regenerated plantlets contain diverse biochemical compounds with important pharmacological properties. Furthermore, the biochemical composition of the two is substantially comparable, indicating their metabolic similarities. The genetic similarity between both plants was confirmed by the presence of monomorphic bands generated using the Start Codon Targeted (SCoT) and Inter Simple Sequence Repeat (ISSR) markers. Morphological variation was studied using scanning electron microscopy (SEM) analysis. SEM observations revealed the uniform arrangements of trichomes and stomata during the in vitro regeneration.