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<p>This study collected the initial material of somatic embryos (SPC explants: single-piece cotyledonary somatic embryos) and regenerated materials <i>via</i> bipolar development from <i>Rosa hybrida</i> ‘J. F. Kennedy’ (RSLA seedling: regenerated seedling with single bud along the longitudinal axis) to be used for transcriptome sequencing, with SPC explants as the control. By analyzing transcriptome data, the study aimed to investigate the characteristics of molecular expression, specifically the metabolism of starch aerobic decomposition during the somatic embryo bipolar development of <i>R. hybrida</i> ‘J. F. Kennedy.’ In addition, the study further elucidated the energy metabolism mechanism underlying the bipolar development of rose somatic embryo. The results showed that a total of 50 differentially expressed genes (DEGs; 19 upregulated and 31 downregulated), related to the starch aerobic decomposition metabolism pathway, were identified in the SPC explants and RSLA seedlings comparison group. Comparative analysis revealed significant differences in the expression of related genes in the cells of the two above materials, whether in the chloroplast starch hydrolysis pathway, the cytoplasmic glycolysis pathway, or the mitochondrial TCA cycle. The corresponding metabolic pathways were distinctly different, with starch hydrolysis reactions more active in the regenerated seedlings, glycolytic reactions more active in the somatic embryo cells, and the TCA cycle reactions having different active pathways in both materials. This research might provide a reference for understanding the molecular mechanisms of the plantlet regeneration approach <i>via</i> bipolar germination in rose somatic embryo from the perspective of carbohydrate metabolism.</p>

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Expression patterns for starch metabolism during somatic embryo bipolar development of Rosa hybrida

  • 期刊论文

  • 发表日期：

    2025年9月4日

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  • Xiaoling Kang,
  • Xiaoxue Hu,
  • Chuanyu Ding,
  • Li Du

摘要

This study collected the initial material of somatic embryos (SPC explants: single-piece cotyledonary somatic embryos) and regenerated materials via bipolar development from Rosa hybrida ‘J. F. Kennedy’ (RSLA seedling: regenerated seedling with single bud along the longitudinal axis) to be used for transcriptome sequencing, with SPC explants as the control. By analyzing transcriptome data, the study aimed to investigate the characteristics of molecular expression, specifically the metabolism of starch aerobic decomposition during the somatic embryo bipolar development of R. hybrida ‘J. F. Kennedy.’ In addition, the study further elucidated the energy metabolism mechanism underlying the bipolar development of rose somatic embryo. The results showed that a total of 50 differentially expressed genes (DEGs; 19 upregulated and 31 downregulated), related to the starch aerobic decomposition metabolism pathway, were identified in the SPC explants and RSLA seedlings comparison group. Comparative analysis revealed significant differences in the expression of related genes in the cells of the two above materials, whether in the chloroplast starch hydrolysis pathway, the cytoplasmic glycolysis pathway, or the mitochondrial TCA cycle. The corresponding metabolic pathways were distinctly different, with starch hydrolysis reactions more active in the regenerated seedlings, glycolytic reactions more active in the somatic embryo cells, and the TCA cycle reactions having different active pathways in both materials. This research might provide a reference for understanding the molecular mechanisms of the plantlet regeneration approach via bipolar germination in rose somatic embryo from the perspective of carbohydrate metabolism.