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<p>Heteroploid plants of <i>Anthurium andreanum</i> 'Tropical Red' were successfully induced by treating diploid callus with colchicine. Callus induction and proliferation were achieved on half-strength MS medium supplemented with 1.0&#xa0;mg L⁻<sup>1</sup> BA and 0.5&#xa0;mg L⁻<sup>1</sup> 2,4-D. The resulting callus were exposed to colchicine at three concentrations (0.1%, 0.2%, and 0.3%) for durations of 3, 5, and 7&#xa0;h. Following treatment, callus were transferred to shoot induction medium, and ploidy levels were determined using flow cytometry. Tetraploid plants were obtained in all treatments with induction rates ranging from 1.20 ± 0.08% to 7.00 ± 0.40%. Increasing colchicine concentration and exposure time significantly reduced explant survival. The optimal treatment was identified as 0.2% colchicine for 7&#xa0;h, which resulted in 6.40 ± 0.14% tetraploid induction, 7.00 ± 0.14% mixoploid formation, and a 50.00% survival rate following shoot multiplication. These results demonstrate the feasibility of <i>in vitro</i>-induced heteroploidy in <i>A. andreanum</i> and provide a foundation for future breeding and germplasm improvement efforts.</p>

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In vitro induction of heteroploidy in Anthurium andreanum Linden

  • 期刊论文

  • 发表日期：

    2025年8月20日

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  • P. K. Murshid Muhammed,
  • Beena Thomas,
  • G. Seeja,
  • K. P. Sindura,
  • A. Adheena Ram,
  • P. K. Fahida

摘要

Heteroploid plants of Anthurium andreanum 'Tropical Red' were successfully induced by treating diploid callus with colchicine. Callus induction and proliferation were achieved on half-strength MS medium supplemented with 1.0 mg L⁻1 BA and 0.5 mg L⁻1 2,4-D. The resulting callus were exposed to colchicine at three concentrations (0.1%, 0.2%, and 0.3%) for durations of 3, 5, and 7 h. Following treatment, callus were transferred to shoot induction medium, and ploidy levels were determined using flow cytometry. Tetraploid plants were obtained in all treatments with induction rates ranging from 1.20 ± 0.08% to 7.00 ± 0.40%. Increasing colchicine concentration and exposure time significantly reduced explant survival. The optimal treatment was identified as 0.2% colchicine for 7 h, which resulted in 6.40 ± 0.14% tetraploid induction, 7.00 ± 0.14% mixoploid formation, and a 50.00% survival rate following shoot multiplication. These results demonstrate the feasibility of in vitro-induced heteroploidy in A. andreanum and provide a foundation for future breeding and germplasm improvement efforts.