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<p><i>Chresta exsucca</i> DC. (<i>Asteraceae</i>) is a species native to Cerrado that stores inulin-type fructans in its underground organs. These water-soluble polysaccharides are valuable in the pharmaceutical and food industries and are crucial in plant responses to abiotic stress. Although several <i>Asteraceae</i> species have been micropropagated, few have been studied for <i>in vitro</i> fructan production. This study aimed to establish the micropropagation protocol of <i>C. exsucca</i> and examine fructan production in the organs of plants cultivated <i>in vitro</i>. Cypselae of <i>C. exsucca</i>, collected from field-grown plants, were disinfested for <i>in vitro</i> germination. The seedlings provided nodal segments that were inoculated in Murashige and Skoog (MS) medium with varying concentrations of 6-benzylaminopurine (BAP) for shoot induction, followed by indole-3-acetic acid (IAA) for rooting. Fructans were analyzed in the organs of micropropagated plants. All cypselae of <i>C. exsucca</i> successfully germinated <i>in vitro</i> after the disinfestation treatment with 1.25% active chlorine followed by inoculation in MS medium without or with 100% of macronutrients. The highest shoot proliferation occurred on MS containing 4.44&#xa0;µM BAP. Root elongation was most pronounced in the absence of IAA or at the lower concentration tested (0.57 and 2.86&#xa0;µM), whereas higher IAA levels inhibited root extension. Plants acclimatized in a 1:1 mixture of commercial substrate and soil from <i>C. exsucca</i>’s natural habitat achieved a 73.3% survival rate. Inulin-type fructans were produced <i>in vitro</i> in roots and calluses but not in other organs. The micropropagation techniques applied were effective in obtaining fructan-producing clones of <i>C. exsucca</i>.</p>

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Micropropagation and in vitro fructan production by Chresta exsucca DC. (Asteraceae)

  • 期刊论文

  • 发表日期：

    2025年6月30日

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  • Dayana Figueiredo Abdalla,
  • Andreia Alves da Costa Silveira,
  • Sérgio Tadeu Sibov,
  • Moemy Gomes de Moraes

摘要

Chresta exsucca DC. (Asteraceae) is a species native to Cerrado that stores inulin-type fructans in its underground organs. These water-soluble polysaccharides are valuable in the pharmaceutical and food industries and are crucial in plant responses to abiotic stress. Although several Asteraceae species have been micropropagated, few have been studied for in vitro fructan production. This study aimed to establish the micropropagation protocol of C. exsucca and examine fructan production in the organs of plants cultivated in vitro. Cypselae of C. exsucca, collected from field-grown plants, were disinfested for in vitro germination. The seedlings provided nodal segments that were inoculated in Murashige and Skoog (MS) medium with varying concentrations of 6-benzylaminopurine (BAP) for shoot induction, followed by indole-3-acetic acid (IAA) for rooting. Fructans were analyzed in the organs of micropropagated plants. All cypselae of C. exsucca successfully germinated in vitro after the disinfestation treatment with 1.25% active chlorine followed by inoculation in MS medium without or with 100% of macronutrients. The highest shoot proliferation occurred on MS containing 4.44 µM BAP. Root elongation was most pronounced in the absence of IAA or at the lower concentration tested (0.57 and 2.86 µM), whereas higher IAA levels inhibited root extension. Plants acclimatized in a 1:1 mixture of commercial substrate and soil from C. exsucca’s natural habitat achieved a 73.3% survival rate. Inulin-type fructans were produced in vitro in roots and calluses but not in other organs. The micropropagation techniques applied were effective in obtaining fructan-producing clones of C. exsucca.