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<p><i>Opuntia ficus-indica</i>, commonly known as prickly pear, holds significant importance within the Cactaceae family. It is broadly cultivated and distributed around the world, generally in arid, semi-arid, and Mediterranean climate zones. It is mostly propagated by seeds, as well as vegetatively through cuttings or grafting. Yet, these methods are still insufficient for large-scale propagation. To handle the main issues associated with these conventional propagation methods, the adoption of plant tissue culture has proved to be a feasible solution. Micropropagation techniques can produce an extremely high number of shoots from a single explant in a short period and within a limited space, which allows a high multiplication rate with homogeneity among propagated materials. However, the success of this technique relies on several factors, such as the type of explant, the nutrient formulation of the culture medium, and the type and amounts of plant growth regulators. This review marks the first comprehensive examination of the <i>in vitro</i> propagation method of areole activation employed for <i>O. ficus-indica</i> by stimulating axillary buds. Additionally, it covers different aspects, including explant selection, sterilization protocols, culture media formulation, plant growth regulators, and the necessary conditions for successful induction and multiplication of axillary buds.</p>

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Micropropagation in vitro of Opuntia ficus-indica through axillary buds’ proliferation: a review

  • 期刊论文

  • 发表日期：

    2025年6月10日

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  • Imane Touaf,
  • Aissam El Finti,
  • Khalid Lagram,
  • Mohammed Amine Serghini,
  • Abdelhamid El Mousadik,
  • Oumaima Bouihate,
  • Imane Boudadi,
  • Soumaya El Merzougui,
  • Ikram Bachti,
  • Rachida El Boullani

摘要

Opuntia ficus-indica, commonly known as prickly pear, holds significant importance within the Cactaceae family. It is broadly cultivated and distributed around the world, generally in arid, semi-arid, and Mediterranean climate zones. It is mostly propagated by seeds, as well as vegetatively through cuttings or grafting. Yet, these methods are still insufficient for large-scale propagation. To handle the main issues associated with these conventional propagation methods, the adoption of plant tissue culture has proved to be a feasible solution. Micropropagation techniques can produce an extremely high number of shoots from a single explant in a short period and within a limited space, which allows a high multiplication rate with homogeneity among propagated materials. However, the success of this technique relies on several factors, such as the type of explant, the nutrient formulation of the culture medium, and the type and amounts of plant growth regulators. This review marks the first comprehensive examination of the in vitro propagation method of areole activation employed for O. ficus-indica by stimulating axillary buds. Additionally, it covers different aspects, including explant selection, sterilization protocols, culture media formulation, plant growth regulators, and the necessary conditions for successful induction and multiplication of axillary buds.