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<p>This pioneering study focuses on assessing the impact of meta-topolin (<i>m</i>T) hormone on the <i>in vitro</i> shoot proliferation, rooting, and genetic fidelity of <i>Rubia cordifolia</i> L. commonly known as Manjistha or Indian Madder to ultimately refine the micropropagation strategy. A significant positive residual effect of optimum <i>m</i>T concentration on <i>in vitro</i> rooting in <i>R. cordifolia</i> was also demonstrated. Sixteen different growth medium combinations were tested, including the basal MS medium supplemented with various concentrations of <i>m</i>T (ranging from 0.05 to 5.0 mg/L) and other cytokinins (BAP, Kn, 2-iP at 1.0 mg/L concentration) for <i>in vitro</i> shoot proliferation. <i>R. cordifolia</i> demonstrated 100% shoot induction across all the tested medium combinations. The concentration of <i>m</i>T significantly influenced shoot production, with the 2.0 mg/L concentration proving most effective, yielding the highest number of shoots (13.17±0.27) and nodes (28.44±0.71) after 16 wk, which further increased to 26.83±0.35 shoots and 48.73±0.70 nodes after 24 wk. Optimal <i>in vitro</i> rooting was achieved with 2.0 mg/L IBA, producing the maximum number of roots (18.50±0.48) after 8 wk. The residual effect of various cytokinins, including <i>m</i>T, on <i>in vitro</i> rooting was also investigated. Micro-shoots multiplied using 2.0 mg/L <i>m</i>T generated the best results, yielding a significantly higher number of roots (20.22±0.62) than micro-shoots multiplied using half basal MS. During acclimatization, all the rooted plants displayed 100% survival. Genetic integrity assessment using 20 ISSR markers demonstrated no discernible differences in the banding patterns between the mother plant and the <i>in vitro</i> regenerated plantlets. This optimized methodology has the potential for large-scale propagation of uniform-quality disease-free madder planting material to not only reduce the pressure on natural resources but also replenish its natural populations.</p>

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Effect of meta-topolin on in vitro shoot multiplication, rooting, and genetic integrity of Rubia cordifolia L

  • 期刊论文

  • 发表日期：

    2025年4月8日

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  • Kunal,
  • Sangita Bansal,
  • Manoj K Sharma,
  • Prachi Pant,
  • Era V Malhotra,
  • Jyoti Kumari,
  • SK Malik

摘要

This pioneering study focuses on assessing the impact of meta-topolin (mT) hormone on the in vitro shoot proliferation, rooting, and genetic fidelity of Rubia cordifolia L. commonly known as Manjistha or Indian Madder to ultimately refine the micropropagation strategy. A significant positive residual effect of optimum mT concentration on in vitro rooting in R. cordifolia was also demonstrated. Sixteen different growth medium combinations were tested, including the basal MS medium supplemented with various concentrations of mT (ranging from 0.05 to 5.0 mg/L) and other cytokinins (BAP, Kn, 2-iP at 1.0 mg/L concentration) for in vitro shoot proliferation. R. cordifolia demonstrated 100% shoot induction across all the tested medium combinations. The concentration of mT significantly influenced shoot production, with the 2.0 mg/L concentration proving most effective, yielding the highest number of shoots (13.17±0.27) and nodes (28.44±0.71) after 16 wk, which further increased to 26.83±0.35 shoots and 48.73±0.70 nodes after 24 wk. Optimal in vitro rooting was achieved with 2.0 mg/L IBA, producing the maximum number of roots (18.50±0.48) after 8 wk. The residual effect of various cytokinins, including mT, on in vitro rooting was also investigated. Micro-shoots multiplied using 2.0 mg/L mT generated the best results, yielding a significantly higher number of roots (20.22±0.62) than micro-shoots multiplied using half basal MS. During acclimatization, all the rooted plants displayed 100% survival. Genetic integrity assessment using 20 ISSR markers demonstrated no discernible differences in the banding patterns between the mother plant and the in vitro regenerated plantlets. This optimized methodology has the potential for large-scale propagation of uniform-quality disease-free madder planting material to not only reduce the pressure on natural resources but also replenish its natural populations.