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<p><i>Dendrobium primulinum</i> Lindl. is a rare and extremely endangered epiphytic <i>Dendrobium</i> with high ornamental and medicinal value. In this study, the effects of Murashige and Skoog (MS) medium strength, types and concentrations of natural organic additives on stem segment regeneration were investigated. Moreover, the one-step seedling medium for stem segments was identified using various combinations of plant growth regulators. Subsequently, the apical buds were inoculated into the one-step seedling medium, and the embryogenic callus was successfully obtained. The results indicated that the most suitable basal medium was 1/2 MS supplemented with 40.0&#xa0;g·L<sup>−1</sup> banana puree and 30.0&#xa0;g·L<sup>−1</sup> potato puree. The one-step seedling medium consisted of the basal medium supplemented with 1.0&#xa0;mg·L<sup>−1</sup> 6-BA, 0.5&#xa0;mg·L<sup>−1</sup> KT, and 2.0&#xa0;mg·L<sup>−1</sup> NAA, and the proliferation coefficient in this medium was increased to 8.42. When subcultured on the above medium, the regeneration pathway of apical buds mirrored that of the stem segments (basal stem cluster bud); however, the proliferation coefficient (4.7) was significantly lower than that of the stem segments. Additionally, 4.7% of the apical buds showed embryogenic callus at the base, which could be further cultured to form protocorm-like bodies that subsequently developed into intact plantlets, with a proliferation coefficient of about 20.0. The survival rate of transplanted seedlings following acclimation can reach 100%. This study achieved the plant regeneration of <i>D. primulinum</i> through both direct and indirect organogenesis pathways. The direct organogenesis pathway shortens the regeneration cycle, enhances reproductive efficiency, and effectively preserves parent plant characteristics. Conversely, the indirect organogenesis pathway prolongs the regeneration cycle but achieves an exceptionally high proliferation coefficient. In practical applications, these two pathways can be proportionally combined based on specific conditions to produce high-yield and high-quality <i>D. primulinum</i> plantlets.</p>

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Establishment of an efficient in vitro regeneration protocol for Dendrobium primulinum Lindl. by one-step seedling formation

  • 期刊论文

  • 发表日期：

    2025年4月24日

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  • Cheng-Li Liu,
  • Jie-Mei Deng,
  • Shun Peng,
  • Heng-Yu Huang,
  • Fu-Rong Xu

摘要

Dendrobium primulinum Lindl. is a rare and extremely endangered epiphytic Dendrobium with high ornamental and medicinal value. In this study, the effects of Murashige and Skoog (MS) medium strength, types and concentrations of natural organic additives on stem segment regeneration were investigated. Moreover, the one-step seedling medium for stem segments was identified using various combinations of plant growth regulators. Subsequently, the apical buds were inoculated into the one-step seedling medium, and the embryogenic callus was successfully obtained. The results indicated that the most suitable basal medium was 1/2 MS supplemented with 40.0 g·L−1 banana puree and 30.0 g·L−1 potato puree. The one-step seedling medium consisted of the basal medium supplemented with 1.0 mg·L−1 6-BA, 0.5 mg·L−1 KT, and 2.0 mg·L−1 NAA, and the proliferation coefficient in this medium was increased to 8.42. When subcultured on the above medium, the regeneration pathway of apical buds mirrored that of the stem segments (basal stem cluster bud); however, the proliferation coefficient (4.7) was significantly lower than that of the stem segments. Additionally, 4.7% of the apical buds showed embryogenic callus at the base, which could be further cultured to form protocorm-like bodies that subsequently developed into intact plantlets, with a proliferation coefficient of about 20.0. The survival rate of transplanted seedlings following acclimation can reach 100%. This study achieved the plant regeneration of D. primulinum through both direct and indirect organogenesis pathways. The direct organogenesis pathway shortens the regeneration cycle, enhances reproductive efficiency, and effectively preserves parent plant characteristics. Conversely, the indirect organogenesis pathway prolongs the regeneration cycle but achieves an exceptionally high proliferation coefficient. In practical applications, these two pathways can be proportionally combined based on specific conditions to produce high-yield and high-quality D. primulinum plantlets.