Scholarlink

<p>Pineapple (<i>Ananas comosus</i> (L.) Merr.) is a nourishing tropical fruit known for its distinctive aroma and exquisite taste. The traditional pineapple propagation method exposes the plants to various soil-borne fungal infections, the most persistent of which is <i>Fusarium moniliforme</i>. The current study examines the effect of overexpression of a <i>Somatic Embryogenesis Receptor Kinase</i> (<i>SERK</i>) gene on <i>F. moniliforme</i> infection in pineapple. <i>AcSERK3</i> was the most promising of the three homologues of the pineapple <i>SERK</i> gene family as reported by Pal and Gangopadhyay (<i>Vegetos</i> 35:571–584, 2022). It was anticipated that overexpression of <i>AcSERK3</i> would bring about <i>Fusarium</i>-tolerance in pineapple. Pineapple plants containing the overexpression construct had more copies of <i>AcSERK3</i> transcripts. Histochemical assays and PCR/qRT-PCR studies confirmed that the gene-construct had been integrated effectively into the putative transformed plants. Compared to the wildtype (non-transformed) plantlets, the transformed plantlets displayed remarkable regeneration capacity. When challenged with <i>F. moniliforme</i>, transformed plantlets had a reduction in disease symptoms and decreased fungal transcript accumulation compared to wildtype plantlets. The potential effects of the overexpressed gene on the defensive response of transformed and wildtype pineapple plants to <i>Fusarium</i> infection were also examined by quantifying the plants’ phenolic substances, proline, and defense-related enzymes. This is the first report of a gene overexpressed in pineapple that induces somatic embryogenesis and disease tolerance. The study may pave the way for developing a <i>Fusarium</i>-tolerant cultivar for pineapple growers.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Overexpression of AcSERK3 confers Fusarium-tolerance to transgenic pineapple

  • 期刊论文

  • 发表日期：

    2025年5月20日

查看全文
  • Soumili Pal,
  • Gaurab Gangopadhyay

摘要

Pineapple (Ananas comosus (L.) Merr.) is a nourishing tropical fruit known for its distinctive aroma and exquisite taste. The traditional pineapple propagation method exposes the plants to various soil-borne fungal infections, the most persistent of which is Fusarium moniliforme. The current study examines the effect of overexpression of a Somatic Embryogenesis Receptor Kinase (SERK) gene on F. moniliforme infection in pineapple. AcSERK3 was the most promising of the three homologues of the pineapple SERK gene family as reported by Pal and Gangopadhyay (Vegetos 35:571–584, 2022). It was anticipated that overexpression of AcSERK3 would bring about Fusarium-tolerance in pineapple. Pineapple plants containing the overexpression construct had more copies of AcSERK3 transcripts. Histochemical assays and PCR/qRT-PCR studies confirmed that the gene-construct had been integrated effectively into the putative transformed plants. Compared to the wildtype (non-transformed) plantlets, the transformed plantlets displayed remarkable regeneration capacity. When challenged with F. moniliforme, transformed plantlets had a reduction in disease symptoms and decreased fungal transcript accumulation compared to wildtype plantlets. The potential effects of the overexpressed gene on the defensive response of transformed and wildtype pineapple plants to Fusarium infection were also examined by quantifying the plants’ phenolic substances, proline, and defense-related enzymes. This is the first report of a gene overexpressed in pineapple that induces somatic embryogenesis and disease tolerance. The study may pave the way for developing a Fusarium-tolerant cultivar for pineapple growers.