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<p><i>Ceropegia elegans</i> Wall., a twining subshrub endemic to southwestern India and Sri Lanka, is valuable in medicine and horticulture. Rapid deforestation within the Western Ghats has reduced wild populations, threatening its regeneration. This study aims to optimize shoot induction using cytokinins (benzyladenine [BA] and kinetin [KIN]) and rooting with silver nanoparticles (S-NPs) synthesized using indole-3-acetic acid (IAA) or α-naphthaleneacetic acid (NAA) <i>via</i> silver nitrate (AgNO<sub>3</sub>). IAA and NAA acted as stabilizing agents during nanoparticle synthesis, ensuring uniformity. BA at 2.0&#xa0;mg L<sup>−1</sup> exhibited superior shoots (3.35 ± 0.65) proliferation, while IAA and NAA (0.5 to 1.5&#xa0;mg L<sup>−1</sup>) alone showed limited rooting. However, S-NPs stabilized with IAA (S-IAA) and NAA (S-NAA) significantly improved rooting efficiency in both <i>in vitro</i> and <i>ex vitro</i> studies. The use of silver nanoparticles enhanced auxin stability, bioavailability, and cellular uptake, thereby promoting rooting and flower formation. S-IAA induced a higher number (4.34 ± 0.12) and longer roots (3.13 ± 0.32&#xa0;cm) compared to S-NAA and normal auxin treatments. Additionally, S-IAA significantly promoted flower formation, highlighting its role in reproductive success, which is essential for preserving genetic diversity and enabling long-term sustainability of wild populations. Statistical analysis using ANOVA and Tukey’s <i>post hoc</i> test confirmed significant differences, highlighting the superior performance of S-IAA in rooting and flowering. Characterization of the synthesized nanoparticles (S-IAA and S-NAA) through ultraviolet–visible (UV) spectroscopy, scanning electron microscopic (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) confirmed their successful formation and stability. This study integrates nanotechnology with plant tissue culture to develop sustainable strategies for the propagation and conservation of endangered species.</p>

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Nanotechnology-based efficient micropropagation strategies for Ceropegia elegans Wall.

  • 期刊论文

  • 发表日期：

    2025年5月9日

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  • S. Stephin,
  • A. Gangaprasad

摘要

Ceropegia elegans Wall., a twining subshrub endemic to southwestern India and Sri Lanka, is valuable in medicine and horticulture. Rapid deforestation within the Western Ghats has reduced wild populations, threatening its regeneration. This study aims to optimize shoot induction using cytokinins (benzyladenine [BA] and kinetin [KIN]) and rooting with silver nanoparticles (S-NPs) synthesized using indole-3-acetic acid (IAA) or α-naphthaleneacetic acid (NAA) via silver nitrate (AgNO3). IAA and NAA acted as stabilizing agents during nanoparticle synthesis, ensuring uniformity. BA at 2.0 mg L−1 exhibited superior shoots (3.35 ± 0.65) proliferation, while IAA and NAA (0.5 to 1.5 mg L−1) alone showed limited rooting. However, S-NPs stabilized with IAA (S-IAA) and NAA (S-NAA) significantly improved rooting efficiency in both in vitro and ex vitro studies. The use of silver nanoparticles enhanced auxin stability, bioavailability, and cellular uptake, thereby promoting rooting and flower formation. S-IAA induced a higher number (4.34 ± 0.12) and longer roots (3.13 ± 0.32 cm) compared to S-NAA and normal auxin treatments. Additionally, S-IAA significantly promoted flower formation, highlighting its role in reproductive success, which is essential for preserving genetic diversity and enabling long-term sustainability of wild populations. Statistical analysis using ANOVA and Tukey’s post hoc test confirmed significant differences, highlighting the superior performance of S-IAA in rooting and flowering. Characterization of the synthesized nanoparticles (S-IAA and S-NAA) through ultraviolet–visible (UV) spectroscopy, scanning electron microscopic (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) confirmed their successful formation and stability. This study integrates nanotechnology with plant tissue culture to develop sustainable strategies for the propagation and conservation of endangered species.