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<p><i>Musa acuminata</i> AAA Cavendish cv. ‘Grand Nain’ is susceptible to banana bunchy top virus (BBTV) and exhibits a compatible host–pathogen interaction. The BBTV-Raipur isolate identified in native <i>Musa</i> field plants in Chhattisgarh, India, was collected and described. It was identified as a distinct member of the Pacific-Indian Oceans (PIO) group by a phylogenetic analysis of 29 nucleotide sequences of the BBTV DNA-S coat protein gene. Further, <i>in vitro</i>, an investigation was conducted to understand the altered physiology of the BBTV-infected regenerants in comparison to the healthy <i>Musa</i> regenerants (control) by studying the growth attributes and biochemical stress indicators such as the amount of leaf pigments, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content, catalase (CAT), and ascorbate peroxidase (APX) activity were determined. Morphological parameters such as shoot proliferation, pseudostem height, girth, and leaf area were reduced significantly in the BBTV-infected regenerants, in contrast to the control. Also, the photosynthetic pigment Chl <i>a</i> content was reduced significantly. With the higher accumulation of the oxidative metabolite H<sub>2</sub>O<sub>2</sub>, APX activity was significantly higher, while the activity of the CAT declined in the BBTV-infected regenerants. Thus, the present findings indicated that the presence of the BBTV particles in the <i>Musa</i> host cells leads to accumulation of H<sub>2</sub>O<sub>2</sub> resulting in declined CAT activity and disturbance in the chloroplast, while the APX activity enhances targeting the accumulated H<sub>2</sub>O<sub>2</sub>. Hence, as a consequence of alteration in the functioning of the cells and disruption of normal physiology, <i>in vitro</i> BBTV-infected <i>M. acuminata</i> regenerants exhibit inferior growth and development.</p>

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Insights into stress indicators during compatible interaction between host plant Musa and viral pathogen BBTV: an in vitro study

  • 期刊论文

  • 发表日期：

    2025年4月21日

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  • Smriti Adil,
  • Chetana Dhruw,
  • Ankita Rathi,
  • Afaque Quraishi

摘要

Musa acuminata AAA Cavendish cv. ‘Grand Nain’ is susceptible to banana bunchy top virus (BBTV) and exhibits a compatible host–pathogen interaction. The BBTV-Raipur isolate identified in native Musa field plants in Chhattisgarh, India, was collected and described. It was identified as a distinct member of the Pacific-Indian Oceans (PIO) group by a phylogenetic analysis of 29 nucleotide sequences of the BBTV DNA-S coat protein gene. Further, in vitro, an investigation was conducted to understand the altered physiology of the BBTV-infected regenerants in comparison to the healthy Musa regenerants (control) by studying the growth attributes and biochemical stress indicators such as the amount of leaf pigments, hydrogen peroxide (H2O2) content, catalase (CAT), and ascorbate peroxidase (APX) activity were determined. Morphological parameters such as shoot proliferation, pseudostem height, girth, and leaf area were reduced significantly in the BBTV-infected regenerants, in contrast to the control. Also, the photosynthetic pigment Chl a content was reduced significantly. With the higher accumulation of the oxidative metabolite H2O2, APX activity was significantly higher, while the activity of the CAT declined in the BBTV-infected regenerants. Thus, the present findings indicated that the presence of the BBTV particles in the Musa host cells leads to accumulation of H2O2 resulting in declined CAT activity and disturbance in the chloroplast, while the APX activity enhances targeting the accumulated H2O2. Hence, as a consequence of alteration in the functioning of the cells and disruption of normal physiology, in vitro BBTV-infected M. acuminata regenerants exhibit inferior growth and development.