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<p><i>Eriobotrya japonica</i> (Thunb.) Lindl<i>.</i> is an evergreen tree that holds economic significance. It is cultivated for its fruits, leaves and ornamental properties. Its propagation by seeds and cuttings is inefficient due to high heterozygosity, lengthy juvenile phase and reproductive cycle. This study establishes a direct <i>in vitro</i> propagation technique for the plant from the mother tree. The impact of different zinc oxide nanoparticle (ZnO-NP) concentrations on <i>E. japonica</i>’s <i>in vitro</i> propagation was assessed. Genetic stability of the micropropagated plantlets was also conducted using start codon targeted (SCoT) and sequence-related amplified polymorphism (SRAP) markers to evaluate the protocols’ efficiency. Successful shoot establishment involved 1.0&#xa0;mg L<sup>−1</sup> 6-benzyladenine (BA) and 0.1&#xa0;mg L<sup>−1</sup> α-naphthaleneacetic acid (NAA) supplemented to Murashige and Skoog (MS) medium, yielding 80% growth induction, the highest shoot number (3.72) and length (4.84&#xa0;cm). MS medium with 1.0&#xa0;mg L<sup>−1</sup> BA and 1.0&#xa0;mg L<sup>−1</sup> kinetin (Kin) achieved 95% multiplication and optimal shoot number (5.70). Adding 50.0&#xa0;mg L<sup>−1</sup> ZnO-NPs significantly influenced shoot formation, leading to 100% growth induction, highest shoot number (7.67) and highest shoot length (4.0&#xa0;cm). Rooting using 1.5&#xa0;mg L<sup>−1</sup> indole-3-butyric acid (IBA) in half MS medium achieved 100% rooting response and 85% survival for post-greenhouse acclimatization. Genetic stability analysis showed low degree of polymorphism (3.3%) and high percentage of monomorphic bands (96.7%) between donor and micropropagated plants. Dice’s similarity coefficient displayed high similarity (0.992 to 1.000) between sub-cultured and mother plants. An effective method for <i>in vitro</i> propagation of <i>E. japonica</i> was successfully established. ZnO-NPs significantly influence shoot formation and facilitate the mass production of <i>E. japonica</i> plants with desired characteristics.</p>

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In vitro propagation and assessment of genetic stability in Eriobotrya japonica (Thunb.) Lindl. as affected by zinc oxide nanoparticles (ZnO-NPs)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月17日

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  • Heba El-Sayed Ghareb,
  • Sabha Salman Mustafa,
  • Shafik Darwish Ibrahim,
  • Mohamed Reda Abd Alhady,
  • Mahdia Farid Gabr

摘要

Eriobotrya japonica (Thunb.) Lindl. is an evergreen tree that holds economic significance. It is cultivated for its fruits, leaves and ornamental properties. Its propagation by seeds and cuttings is inefficient due to high heterozygosity, lengthy juvenile phase and reproductive cycle. This study establishes a direct in vitro propagation technique for the plant from the mother tree. The impact of different zinc oxide nanoparticle (ZnO-NP) concentrations on E. japonica’s in vitro propagation was assessed. Genetic stability of the micropropagated plantlets was also conducted using start codon targeted (SCoT) and sequence-related amplified polymorphism (SRAP) markers to evaluate the protocols’ efficiency. Successful shoot establishment involved 1.0 mg L−1 6-benzyladenine (BA) and 0.1 mg L−1 α-naphthaleneacetic acid (NAA) supplemented to Murashige and Skoog (MS) medium, yielding 80% growth induction, the highest shoot number (3.72) and length (4.84 cm). MS medium with 1.0 mg L−1 BA and 1.0 mg L−1 kinetin (Kin) achieved 95% multiplication and optimal shoot number (5.70). Adding 50.0 mg L−1 ZnO-NPs significantly influenced shoot formation, leading to 100% growth induction, highest shoot number (7.67) and highest shoot length (4.0 cm). Rooting using 1.5 mg L−1 indole-3-butyric acid (IBA) in half MS medium achieved 100% rooting response and 85% survival for post-greenhouse acclimatization. Genetic stability analysis showed low degree of polymorphism (3.3%) and high percentage of monomorphic bands (96.7%) between donor and micropropagated plants. Dice’s similarity coefficient displayed high similarity (0.992 to 1.000) between sub-cultured and mother plants. An effective method for in vitro propagation of E. japonica was successfully established. ZnO-NPs significantly influence shoot formation and facilitate the mass production of E. japonica plants with desired characteristics.